不同温度对罗氏核心抗体试剂检测结果的影响  

Effect of Temperature on Detection Results of Roche HBcAb Reagents

作　　者：左武[1] 谢子睿[2] 

机构地区：[1]武汉大学人民医院,湖北省武汉市430060 [2]湖北中医学院

出　　处：《职业与健康》2010年第4期384-385,共2页Occupation and Health

摘　　要：目的探讨不同温度保存条件对罗氏公司核心抗体试剂的影响。方法以反复冻融试剂为试验组,4℃保存试剂为对照组,通过全自动电化学发光免疫分析仪器分别测定20次乙型病毒性肝炎核心抗体COI(Cutoffindex)值,比较二者之间的差异以及分析二者之间是否存在相关性。结果全自动电化学发光法分析发现试验组高值组COI值为2.1325±0.0532,而对照高组为2.4125±0.1008,二者之间差异有统计学意义(P<0.01),但二者之间不存在正相关性(r2=-0.0676,P>0.05)。试验组低值组COI值为0.5152±0.0057,而对照低组为0.7313±0.0116,二者之间差异亦有统计学意义(P<0.01),且二者之间存在正相关性(r2=0.5567,P<0.05)。结论不同温度条件下保存的罗氏公司核心抗体试剂对实验结果影响较大,试剂不适合保存在0℃以下,且不能反复冻融。[ Objective] To discuss the effect of different storage temperature for Roche HBcAb reagents. [ Methods ] The reagents of experimental group were treated with repeated freezing and thawing, the reagents of control group were stored at 4℃. The COI（ cutoff index） value of HBcA of two groups were determined by automatic electrochemiluminescence analyzer for 20 times respectively, the differences and the correlation between two groups were analyzed. [ Results] The results showed that the CO1 value of high-value group in the experimental group was 2. 1325 ±0.0532, that in the control group was 2. 4125 ± 0. 1008, and the difference between two groups was significant （ P 〈 0.01 ） , but there was no positive correlation （ r^2 = - 0. 0676, P 〉 0.05 ）. The COl value of low-val- ue group in the experimental group and the control group was 0.5152 ± 0. 0057 and 0.7313 ± 0.0116 respectively, the difference between two groups was significant （ P 〈 0.01 ）, and there was positive correlation （r^2 = 0.5567, P 〈 0.05 ）. [ Conclusion] The effect of different storage temperature for Roche HBcAb reagents is obvious. The reagents should not be stored below 0℃, and should be avoid repeated freezing and thawing.

关 键 词：乙型肝炎 核心抗体 全自动免疫发光分析仪 

分 类 号：R446.62[医药卫生—诊断学]