两种温病湿热证模型的TNF-α与TG水平研究  

The Study of changes of the TNF-α and TG in the two kinds of models of Damp-heat Syndrome

作　　者：汤朝晖[1] 苏丽贤[1] 李华锋[2] 罗炳德[1] 林培政[2] 

机构地区：[1]南方医科大学,广东广州510515 [2]广州中医药大学,广东广州510006

出　　处：《中医药信息》2010年第1期14-16,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金-广东省联合基金(U0632009)

摘　　要：目的:通过对小鼠肝炎病毒(MHV-A59毒株)与大肠杆菌导致的两种小鼠温病湿热证模型TNF-α与TG水平的研究,从炎症因子和血脂水平的角度评价两种温病湿热模型特征;方法:将18只小鼠随机分为正常组、病毒组、肠菌组,造模成功后分别取小鼠血液和提取血清,用ELISA法和磷酸甘油氧化法分别测试各组的TNF-α与TG水平,数据分析采用单因素方差分析,组间用q检验,两组间采用t检验,P<0.05为显著性差异,P<0.01为非常显著性差异。结果:病毒组与肠菌组血清TNF-α水平明显增高,且病毒组比肠菌组升高显著;两种模型小鼠TG水平虽有升高但组间变化无差别。结论:"内湿"的形成可能主要与肥甘饮食、湿热外环境有关,MHV-A59作为生物致病因子所致的湿热证模型炎症反应更加剧烈。Objective：To evaluate and compare the two kinds of the mouse models of Damp - heat syndrome caused by the mouse hepatitis virus（ MHV -A59 strain）and E. coli through studying the changes of the TNF -α and TG in these two kinds of mouse models;Methods：Randomly divided 18 mouse into three groups ：control group, virus caused model group and E. coli caused model group. The blood and serum was distilled from the mouse after the models had been established. Then the level of TNF -α and TG were tested by the ELISA and Glycerophosphate oxidation respectively. Last the statistics would be analyzed through one - way anova, q test and t test. Results ： The level of TNF-α of the virus and E. coli caused model group were increased obviously, moreover,the level of the virus caused model group was much higher than the E. colis： There were no difference in the level of the TG between these two groups. Conclusion：The formation of wet in the body may have the relationship with the fat diet and the hot and humid environment. Compared with the E. coli, the model caused by MHV -A59 may have a more severe inflammation.

关 键 词：湿热模型 温病 MHV—A59 大肠杆菌 TNF-Α TG 

分 类 号：R254[医药卫生—中医内科学]