复方黄连制剂对动脉钙化的防治作用  

Effect of Compound Rhizoma Coptidis on artery calcification in rats treated with warfarin and vitamin D

作　　者：方欣[1] 王斌[1] 李伟[1] 管思明[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院老年病科,湖北武汉430022

出　　处：《中成药》2010年第1期22-25,共4页Chinese Traditional Patent Medicine

摘　　要：目的:研究复方黄连制剂(黄连、黄芩、黄柏、栀子和甘草)对华法令和维生素D3诱发大鼠血管中膜钙化的干预效应。方法:SD大鼠40只,随机分成对照组、钙化组、复方黄连制剂预防组、复方黄连制剂治疗组。钙化组与药物干预组均给予华法令和维生素D3诱导大鼠血管钙化模型,复方黄连制剂预防组在制作模型前3天给予复方黄连制剂,治疗组则在模型制作完成后给予复方黄连制剂。对照组给予生理盐水。4周后,比较各组Von Kossa染色及血管组织中钙含量,以及血管壁骨保护素(OPG)mRNA和蛋白表达。结果:32只大鼠最终进入结果分析。与对照组比较,钙化组Von Kossa染色显示血管中膜有明显钙沉积,血管组织中钙含量显著增高,分别为(608.32±42.29)μg/g,(1139.47±230.03)μg/g;血管壁OPGmRNA和蛋白表达则明显减少。预防及治疗应用复方黄连制剂,血管中膜钙化明显改善,血管壁钙含量显著降低,分别为(854.77±12.99)μg/g,(875.78±27.23)μg/g;血管壁OPGmRNA和蛋白也显著增加。与钙化组比较,差异均有统计学意义(P<0.05)。结论:预防或治疗使用复方黄连制剂可能通过增加血管壁骨保护素水平,明显改善血管中膜钙化。AIM： To investigate the effects of Compound Rhizoma Coptidis （Rhizoma coptidis, Radix suctellariae, Cortex phellodendri Chinensis, Fruetus gardeniae and Radix et Rhizoma glycyrrhizae） on vascular calcification in rats treated with warfarin and vitamin D3. METHODS： Thirty-two male SD rats were assigned randomly into control group, calcified group, Compound Rhizoma Coptidis prevented group and treated group. The later three groups were treated with warfarin, and subcutaneously injected with vitamin KI and vitamin D3 for one week to induce extensive calcification of the aorta. Compound Rhizoma Coptidis was given before the first warfarin dose in prevented group and the drug was given after the modeling in the treated group. The control group was treated with normal saline. The calcification in the aorta was analyzed and osteoprotegerin （OPG） mRNA and protein were determined using histomorphometry, RT-PCR and immunohistochemistry after 4 weeks of drug intervention. RESULTS ： The results of 32 rats was analyzed compared to the control group, the area of darkly stained regions by Von Kossa staining and the level of calcium content in aorta wall increased significantly [ （608.32±42.29） μg/g vs （ 1139.47 ± 230.03） μg/g, P 〈 0.05 ]. The OPGmRNA and protein decreased in aortic sections. No artery calcification could be detected in Compound Rhizoma Coptidis prevented group and a little artery calcification could be detected in Compound Rhizoma Coptidis treated group. The level of calcium content in aorta wall significantly lower[ （854.77± 12.99） μg/g, （875.78 ±27.23） μg/g. The expresion of OPGmRNA and the protein signifi-eantly increased in Compound Rhizoma Coptidis prevented and treated groups. CONCLUSION： Compound Rhizoma Coptidis may prevent or regress the vascular calcifiation, that seems dependent on the upregression of aortic OPG levels.

关 键 词：复方黄连 血管钙化 骨保护素 大鼠 

分 类 号：R285.6[医药卫生—中药学]