锡类散对原代培养的小鼠口腔成纤维细胞与创面愈合相关功能的影响(英文)  

Effect of Xilei Powder on primary cultured fibroblast functions related to wound healing

作　　者：代剑平[1] 陈钧[1] 贝宇飞[1] 韩邦兴[1] 周小坤[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《中成药》2010年第1期30-35,共6页Chinese Traditional Patent Medicine

摘　　要：目的:研究锡类散对小鼠口腔成纤维细胞的影响,探索其促伤口愈合的机制。方法:采用MTT法、氯胺-T法、明胶酶谱分析和酶联免疫反应测定锡类散对小鼠口腔成纤维细胞的增殖、群体倍增时间、胶原、金属蛋白酶-2,9(MMP-2,9)、基质金属蛋白酶抑制因子-1(TIMP-1)的影响。结果:锡类散水提物能以时间和浓度依赖性方式促进小鼠口腔成纤维细胞生长,经水提物(125μg/mL)处理后,细胞群体倍增时间为33.8h,并且胶原积累显著增加,其水提物和醇提物都能显著(P<0.05)增加MMP-2活性,同时极显著(P<0.01)提高TIMP-1的合成。结论:锡类散通过促进成纤维细胞分裂、胶原沉积和TIMP-1的产生而激活成纤维细胞,促伤口愈合。AIM： Xilei Powder, a traditional Chinese prescription, has been used to treat wounds for hundreds of years, but the mechanism has not been fully understood. METHODS ： The effects of Xilei Powder on fibroblast proliferation, collagen accumulation, matrix metalloproteinases-2,9 （MMP-2,9） activities and tissue inhibitor of metalloproteinase 1 （TIMP-1） production were investigated by MTT, chloramine T method, gelatin zymography and enzyme-linked immunosorbent assays （ELISA）, respectively. RESULTS： The aqueous extract of Xilei Powder significantly promoted fibroblasts proliferation in a time and concentration manner, the population doubling time （ 125 μg/mL） was 33.8 h, it also significantly （ P 〈 0.05 ） promoted collagen production. Both of the aqueous and alcoholic extracts could significantly （P 〈 0.05 ） increase MMP-2 activity, and also very significantly （P 〈 0.01 ） promote TIMP-1 production. CONCLUSION： Xilei Powder could promote fibroblasts proliferation, collagen and TIMP-1 production, this might be parts of mechanism to promote wound healing.

关 键 词：胶原合成 成纤维细胞 金属蛋白酶-2 9 基质金属蛋白酶抑制因子-1 伤口愈合 

分 类 号：R944.27[医药卫生—药剂学]