HPLC同时测定双黄连粉针剂中5种成分的含量  

Simultaneous determination of five components in Shuanghuanglian Powder for Injection by HPLC

作　　者：孙永慧[1] 李文春 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040 [2]哈药集团中药有限公司研发中心,黑龙江哈尔滨150078 [3]黑龙江中医药大学

出　　处：《中成药》2010年第1期65-68,共4页Chinese Traditional Patent Medicine

摘　　要：目的:建立同时测定双黄连粉针剂(金银花、连翘、黄芩)中绿原酸、咖啡酸、连翘酯苷A、连翘苷和黄芩苷5种成分含量的高效液相色谱方法。方法:采用Phenomenex Luna C18柱(250mm×4.6mm,5μm),甲醇-0.2%磷酸溶液为流动相梯度洗脱,流速为1.0mL/min,检测波长为多波长检测。结果:绿原酸、咖啡酸、连翘酯苷A、连翘苷和黄芩苷分别在0.204～2.04μg、0.0102～0.102μg、0.186～1.86μg、0.0585～0.585μg、2.52～25.2μg范围内线性关系良好(r=0.9997、0.9994、0.9998、0.9998、0.9999),5种成分平均回收率均符合含量测定要求。结论:本法简单易行,结果准确可靠,可有效地用于双黄连粉针剂的质量控制。AIM： To establish a HPLC method for simultaneously determining chlorogenic acid, caffeic acid, forsythin, forsythoside A and baicalin in Shuanghuanglian Powder for Injection （Flos Lonicerae japonicae, Fructus Forsythiae, Radix Scutellariae, etc. ）. METHODS： HPLC assay was carried out on a Phenomenex Luna Cls column （250 mm ×4.6 mm, 5μm）. The mobile phase was methanol-0.2% phosphoric acid solution as gradient elation. The flow rate was 1.0 mL/min. The detection wavelength was set as multi-wavelength detection. RESULTS： The linear ranges of chlorogenic acid, caffeic acid, forsythin, forsythoside A and baiealin fell within the range of 0. 204-2.04 μg,0. 010 2-0. 102 μg,0. 186-1.86 μg,0. 058 5-0. 585μg and 2.52-25.2 μg, respectively. The five components showed good linear correlations （ r = 0. 999 7,0.999 4,0. 999 8,0. 999 8,0. 999 9）. The average reeoveries of five eomponents were in accordance with the determination requirements. CONCLUSION ： The method is simple, quick, reliable, accurate and very suitable to the quality control of Shuanghuanglian Powder for Injeetion.

关 键 词：HPLC 双黄连粉针剂 含量测定 

分 类 号：R927.2[医药卫生—药学]