HPLC同时测定珍珠菜提取物中芦丁和柚皮素-7-O-葡萄糖苷的含量  

Simultaneous determination of rutin and naringenin-7-o-glucoside in extraction of Lysimachia clethroide Duby by HPLC

作　　者：潘海敏[1] 游本刚[1] 唐丽华[1] 李新章[1] 杨世林[1] 

机构地区：[1]苏州大学药学院,江苏苏州215123

出　　处：《中成药》2010年第1期106-109,共4页Chinese Traditional Patent Medicine

基　　金：苏州市社会发展科技项目(SSY0703);苏州大学医学发展基金项(EE132609);国家科技支撑计划子课题(2006BAI06A01-01);国家自然科学基金项目(30873362)

摘　　要：目的:建立同时测定珍珠菜中芦丁和柚皮素-7-O-葡萄糖苷含量的高效液相色谱方法。方法:以Diamonsil C18柱(4.6mm×250mm,5μm)为色谱柱,流动相采用乙腈(A)-0.1%磷酸(B)梯度洗脱:0～18min,83%～80%B;18～30min,80%～86%B。芦丁和柚皮素-7-O-葡萄糖苷的检测波长分别为254nm和281nm,流速为1.0mL/min,柱温为35℃,进样量为20μL。结果:用梯度洗脱得到了较好的分离效果,芦丁在1.00～48.00μg/mL之间线性关系良好,r=0.9993;柚皮素-7-O-葡萄糖苷在0.64～40.72μg/mL之间线性关系良好,r=0.9998。结论:该方法准确,简便,可用于珍珠菜中芦丁和柚皮素-7-O-葡萄糖苷含量的同时测定。AIM:To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS:Using Diamonsil C_ 18(4.6 mm ×250 mm,5μm)as analytical column.The mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid(B)with gradient elution:0~18 min,83%~80%B;18~30 min,80%~86%B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min;Column temperature was at 35 ℃.RESULTS :A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00~48.00 μg/mL(r=0.999 3)and 0.64~40.72 μg/mL(r=0.999 8),respectively.CONCLUSION:The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.

关 键 词：HPLC 珍珠菜 芦丁 柚皮素-7-O-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]