肝素减轻内毒素致大鼠急性肺损伤的实验研究  

Study about heparin reduces acute lung injury of rats induced by endotoxin

作　　者：王涛[1] 潘华[2] 刘红[2] 任雪燕[1] 马京[1] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2010年第1期43-45,共3页Ningxia Medical Journal

摘　　要：目的探讨不同剂量肝素减轻内毒素(LPS)诱导大鼠急性肺损伤(ALI)的作用。方法股静脉注射LPS5mg/kg建立大鼠ALI模型。50只雄性SD大鼠随机分为5组:对照组(A组);LPS组(B组);C1-C3组为肝素+LPS组,分别于LPS注入即刻静注肝素250u/kg,500u/kg,1000u/kg,每组10只。于LPS注射4h后行血气检测并股动脉放血处死大鼠,取肺组织测定肺组织湿/干重比、肺组织丙二醛(MDA),取支气管肺泡灌洗液(BALF)检测肿瘤坏死因子-a(TNF-a)水平和蛋白浓度,光镜下观察肺组织形态学变化。结果B组肺湿/干重比、肺组织MDA、BALF中TNF-a水平和蛋白浓度显著增高;PaO2、PaCO2、BE值明显降低;光镜下肺组织损伤程度增加。C1-C3组各项指标均较内毒素组减轻,但随着剂量增大出血并发症增加。结论肝素早期给药可以减轻内毒素致急性肺损伤,250u/kg为最佳治疗剂量。Objective To investigate the effect of different dose of heparin reduces acute lung injury in rats induced by Lipopolysaecharide （LPS）. Methods LPS （5mg.kg^-1 ） was infused by femoral vein to reproduce ALI model in rats. Fifty male SD rats were randomly divided into five groups with 10 animals in each group： in group C1 -C3 LPS 5mg.kg^-1 and heparin 250u.kg^-1, 500u.kg^-1 and 1000u .kg^-1 was infused by femoral vein simultaneously; group B received LPS 5 mg/kg; group C served as control one by giving the same volume of saline. At 4 h after LPS was given, and then the animals were killed. The pathological manifestation of lungs were observed, and the lung W/D ratio was calculated, and the concentration of malondialdehyde （MDA） in lung was detected, the concentration of TNF - a and protein in BALF were detected. Results Obvious ALl pathological manifestation in the lungs of B group rats were observed. Comparing with A group, the lung W/D ratio and concentration of TNF - a and protein in BALF significantly increased in B group （ P 〈 0.01 ） as well as the concentration of MDA activity in lung （ P 〈 0.01 ）. Comparing with LPS group, the pathological manifestation in the lung and the lung W/D ratio and concentration of TNF - a and protein in BALF and the concentration MDA decreased in C1 - C3 group （ P 〈 0.05 ）. However, bleeding complications increased with the dose of heparin increased. Conclution Early treatment of heparin can provide evident effects on ALl in rats, 250u.kg^- 1 was the best treatment dose.

关 键 词：肝素 急性肺损伤 内毒素 大鼠 

分 类 号：R563[医药卫生—呼吸系统]