陕麦139抗条锈病基因遗传分析  

Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

作　　者：张宏[1] 任志龙[1] 胡银岗[1] 王长有[1] 吉万全[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《作物学报》2010年第1期109-114,共6页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2006CB708208);陕西省自然科学基金(2007C111)项目资助

摘　　要：利用常规遗传和单缺体遗传分析方法,研究了小麦抗条锈病新种质陕麦139中抗病基因的遗传方式。结果表明,陕麦139×辉县红和陕麦139×阿勃两组合F1植株对条中32表现近免疫。F2群体对条中32抗性调查表明,陕麦139×阿勃组合和陕麦139×辉县红组合的抗感比例分别为203:16和210:13,经卡方检验,抗感分离比符合15:1(χ2值分别为0.26和0.02,χ20.05,1=3.84),说明陕麦139所含抗性基因对条中32的抗性受2对独立遗传显性位点控制。21个单缺体组合的F2群体苗期室内接种条中32的抗性分离调查结果表明,阿勃1BN×陕麦139组合抗感分离比例为75:0(χ2=4.65,χ20.05,1=3.84),阿勃2DN×陕麦139组合抗感分离比例为132:2(χ2=4.40,χ20.05,1=3.84),远远偏离15:1,其余19个组合的抗感分离比例经卡方测验均符合15:1。表明该抗条锈病基因位于1B和2D染色体,暂被分别命名为YrSM139-1B和YrSM139-2D。利用284对SSR引物检测F2群体的抗感池和单株,发现YrSM139-1B与SSR标记Xgwm273紧密连锁,即该标记可作为YrSM139-1B抗条锈病基因的标记。利用Xgwm273对陕麦139的亲本分析表明,YrSM139-1B抗条锈病基因来自野生二粒小麦AS846。Stripe rust, caused by Puccinia striiformis West. f. sp. tritici, is one of the most damaging diseases of wheat （Triticum aestivum L.） worldwide. Application of resistant varieties has been considered as the most economical and environment-friendly approach to control the disease. Resistance to stripe rust is frequently overcome by the pathogen due to the emergence of new virulent races. Wheat cultivar Shaanmai 139 is resistant to all current Chinese epidemic races of P. striiformis f. sp. tritici, CYR29 CYR30, CYR31, CYR32, and CYR33. Based on the monosomic genetic analysis, here, we reported the characterization and mo- lecular marker of stripe rust resistance genes to CYR32 in Shaanmai 139. We screened 442 F2 plants derived from two crosses between Shaanmai 139 and two susceptible cultivars Abbondanza and Huixianhong, respectively. The results showed the ratios of the resistant to the susceptible of F2 population from Shaanmai 139x.Abbondanza and Shaanmai 139xHuixianhong were 203：16 and 210：13 respectively, corresponding to 15：1 （χ^2 is 0.26 and 0.02, χ^20.05. 1 = 3.84）. Monosomic analysis showed the ratios deviated from 15：1 in the F2 plants of Shaanmai 139xAbbondaza 1BN and Shaanmai 139xAbbondaza 2DN, while the others ratios corresponded to 15：1. Thus, two resistance loci in Shaanmai 139 were found on chromosomes IB and 2D, tentatively designated YrSM139-1B and YrSM139-2D, respectively, according to the analysis of 21 monosomic and nullsomic lines of the cultivar Abbondanza and SSR mapping results. One SSR marker, Xgwm273, was closely linked to YrSM139-1B in the F2 population. SSR mapping results showed that YrSM139-1B derived from a T. dicoccoides accession, AS846.

关 键 词：小麦 抗病基因 条锈病 遗传分析 

分 类 号：S512.1[农业科学—作物学] S511