表达谱芯片对灰绿藜和水稻NHX1基因在转基因拟南芥中表达差异的研究  

Study on NHX1 Gene Functional Comparisons between Chenopodium glaucum and Oriza sativa Using Affymetrix Gene Chips in Transgenic Arabidopsis

作　　者：郭继娜[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2010年第1期1-6,F0003,共7页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技重大专项(200731138-3);国家"973"计划前期研究专项(2009CB126006)

摘　　要：【目的】基因芯片作为高通量、高效率的DNA分析技术,已被广泛地用于植物抗逆性功能基因的分析之中。为探讨盐生植物和甜土植物NHX1基因调控盐应答过程和亲水性C端的功能研究提供借鉴,进一步揭示盐生植物和甜土植物耐盐机制的差异,为植物耐盐机理的实际应用提供依据。【方法】利用Affymetrix拟南芥表达谱芯片对过表达盐生植物灰绿藜和甜土植物水稻NHX1基因及缺失部分C端基因的转基因拟南芥进行基因表达差异的分析。【结果】在盐胁迫的转基因拟南芥组织中,筛选出在四组转基因型拟南芥中共同差异表达的基因有394条,占筛选基因总数的1.64%,其中上调表达的基因有177条,下调表达的基因有217条。将这些差异基因初步分为12类,包括非生物性与生物性刺激的应答过程、胁迫应答过程、电子转移和能量途径、信号转导和转录等功能类别。【结论】表明植物耐盐过程是一个多基因参与的复杂的变化过程,涉及到许多相关基因的变化。联系两两比较散点图和聚类分析结果从理论上说明,转基因拟南芥cgNHX1,cgNHX1dc,osNHX1和osNHX1dc耐盐性有依次减弱的趋势。[Objective and Method]The gene chip is a DNA analysis technology of high throughput and high efficiency which has been widely used in analysis of resistant function. In this stud）＇, using Affymetrix Arabidopsis whole - genome expression gene chip, the differential expression analysis of gene was carried out with the transgenic A. thaliana . [Result] In the transgenic Arabidopsis tissues under salt stress, 394 co-expression genes were selected from the four groups, they accounted for 1.64% of the total number of genes, in which there were 177 up - regulated expression genes and 217 downregulated expression genes. These genes were initially divided into 12 gene categories, including response to abiotic or biotic stimuli, response to stress , electron transport or energy pathways, signal transduction and transcription, and so on. Our results show that the process of plant salttolerant is a muhi- gene changed complex process that involves many changes of variety related genes. [Conclusion]The results of comparison scatters and cluster analysis illuminate that the salt tolerance of transgenie Arabidopsis cgNHY1, cgNHX1dc, osNHX1 and osNHX 1 dc have successively weakened. Our study will contribute to find the differences between haluphytic and glycophytic species in the mechanism of plant salt tolerance and the function analalysis of hydrophilic C - terminal, provide a favorable evidence for the study of the mechanism of plant salt - tolerance between halophytic and glycophytic species, and offer new methodology in stud）, the mechanism for salt - tolerant plants, provide a basis for practical applications of salttolerant mechanism in plants.

关 键 词：表达谱芯片 灰绿藜 水稻 NHX1 植物耐盐 

分 类 号：S511[农业科学—作物学] S188