大豆农杆菌子叶节转化菌株适宜生长时期及浸染浓度的研究  

Optimization study on strain cultivation period and infectious concentration on soybean cotyledonary node via Agrobacterium-mediated transformation system

作　　者：武小霞[1] 李静[1] 刘伟婷[2] 刘珊珊[1] 王志坤[1] 李文滨[1] 

机构地区：[1]大豆生物学教育部重点实验室,东北农业大学农学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《东北农业大学学报》2010年第1期1-6,共6页Journal of Northeast Agricultural University

基　　金：转基因生物新品种培育重大专项(2008ZX08004-002);哈尔滨市科技创新人才研究专项资金项目(2008RFQXN013);东北农业大学博士后基金与博士启动基金

摘　　要：在大豆子叶节转化过程中,农杆菌侵染受体材料受多种因素的影响。文章对农杆菌侵染受体的菌株生长时期和浸染浓度进行了探讨。结果表明,菌株生长时期OD600=0.6和菌液的浸染浓度为OD600=0.5时,子叶节抗性芽率最高,利用此结果筛选出对农杆菌具有高度的易感性大豆品种3个,为大豆转化效率的提高奠定了基础。In soybean cotyledonary node transformation, Agrobacterium infection was influenced by many factors, The cultivation period of Agrobacterium strain and infectious concentration were studied in the research. The results showed that the highest rate of resistant buds was obtained when strain cultivation concentration was 0.6 at OD600, and the proper infectious concentration was 0.5 at OD600. Seven cultivars of soybean in north China were also screened for high susceptibility to Agrobacterium and three genotypes were found to be suitable for transformation. This research lays a good foundation for improvement of soybean transformation.

关 键 词：大豆 子叶节 农杆菌 菌株生长时期 浸染浓度 

分 类 号：S565.1[农业科学—作物学] Q94[生物学—植物学]