荧光分光光度法测定大鼠不同脑区单胺类神经递质  

Content determination of monoamine neurotransmitter in different encephalic regions in rat by fluorospectrophotometry

作　　者：张燕[1] 王洪斌[1] 杨海霞[2] 陈清[1] 赵丽[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2010年第1期93-96,共4页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(30671552);黑龙江省自然科学基金重点项目(ZJN0503-01)

摘　　要：介绍一种测定大鼠不同脑区单胺类神经递质的方法。采用荧光分光光度法同时测定大鼠不同脑区NE、DA和5-HT三种单胺类神经递质的含量。结果表明,荧光强度与浓度呈线性相关,NE、DA和5-HT的检测回收率分别为102.2%、95.4%和102.8%。该方法快速、灵敏、重复性较高,可为临床监测及科研提供参考。The paper introduced a method for the determination of the monoamine neurotrans-mitter in different encephalic regions in rat. The contents of three monoamine neurotransmitter NE,L-DA and 5-HT in different encephalic regions in rat were measured by fluorospectrophotometry. The results showed that a linear dependence was presented between the fluorescence intensity and the concentration, the recovery rates of the NE, DA and 5-HT were 102.2%, 95.4% and 102.8%, respectively. Thus, this method is rapid, sensitive and higher reproducibility and supplies a reference for the clinic determination and scientific research.

关 键 词：荧光分光光度法 不同脑区 单胺类神经递质 

分 类 号：R285.5[医药卫生—中药学]