猪IL-15与PRRS病毒GP5基因核酸疫苗免疫效力  

Preliminary evaluation on immunization of porcine IL-15 and PRRSV GP5 genes in mice

作　　者：石娜[1] 尹杰超[2] Dante Zarlenga 李广兴[1] 任晓峰[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030 [3]美国农业部农业研究所中心牛功能基因组和动物寄生虫病实验室,马里兰州20705

出　　处：《东北农业大学学报》2010年第1期97-102,共6页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(30700590;30972937);黑龙江省自然科学基金重点项目(ZJN0702-01)

摘　　要：通过分子克隆技术构建了表达猪白细胞介素(IL)-15及猪繁殖呼吸综合症(PRRSV)病毒GP5基因重组核酸疫苗。以小鼠为试验模型,利用猪IL-15作为分子佐剂并检测其对表达PRRS病毒GP5基因核酸疫苗的免疫增强作用。结果表明,猪IL-15与GP5试验组小鼠抗体水平和T淋巴细胞亚群数量均高于PBS对照组及GP5基因单独免疫组,表明猪IL-15对核酸疫苗可有效增强PRRS病毒GP5基因的免疫效果,证实了其佐剂效应。The recombinant plasmids containing either PRRSV GP5 gene or porcine IL-15 gene were constructed by using molecular cloning techniques, and mice were used as animal models. The preliminary evaluation on the effect of IL-15 on PRRSV GP5 gene vaccination was performed through inoculating the vaccines to mice. The results showed that both antibody level, the number of T lymphocytes, and the peripheral blood of immunized mice were higher than those of the control group. IL-15 and GP5 combined group was better than GP5 group in terms of the detected indexes, which indicated that porcine IL-15 displayed an adjuvant effect.

关 键 词：猪IL-15 PRRSV GP5基因 疫苗 

分 类 号：S828[农业科学—畜牧学] Q786[农业科学—畜牧兽医]