注射用水溶性维生素中生物素含量测定方法改进  

Improve content determination method of biotin in water-soluble vitamins for injection

作　　者：杜碧莹[1] 刘丽芳[1] 

机构地区：[1]广州市药品检验所,510160

出　　处：《临床合理用药杂志》2010年第2期11-13,共3页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的建立高效液相色谱法,改进注射用水溶性维生素中生物素的含量测定方法。方法色谱柱为COSMOSILC18(250×4.6mm,5μm),以缓冲液(取0.5g高氯酸钠,加入1ml磷酸,用水稀释至1000ml)为流动相A,乙腈-甲醇(1:2)为流动相B,以流速1.5ml/min进行梯度洗脱;柱温为30℃;检测波长为200nm。结果生物素在1.5～4.5μg/mg的浓度范围内呈线性关系,r=0.9995(n=5)。平均回收率为101.0%,RSD为0.6%(n=9)。结论本方法简便、准确,不受其他成分的干扰,可用于测定注射用水溶性维生素中生物素的含量。Objective To establish a HPLC method, to improve the content determination of biotin in water-soluble vitamins for injection. Methods COSMOSIL C18 （250mm × 4.6mm,5μm）was used as the column. Mobile phase A：buffer solution, Mobile phase B ：aeetonitrile-methanol（1：2）. The flow rate was 1.5ml/min, following the gradient elution. The column temperature was 30℃ and the detection wavelength was 200nm. Results Biotin presented a good linear in the range of 1.5 ～ 4.5μg/mg,r =0.9995（n =5）. The average recovery was 101.0% ,RSD =0.6% （n =9）. Conclusion The present method is simple and accurate for content determination of biotin in water-soluble vitamins for injection, and it also has the advantage of preventing the interference from other compounds.

关 键 词：注射用水溶性维生素 生物素 含量测定 

分 类 号：R927.2[医药卫生—药学]