抗血栓药蕲蛇酶对高血糖大鼠脑中动脉缺血/再灌注后脑损伤的改善作用  

Reduction of brain injury by antithrombotic agent acutobin after middlecerebral artery ischemia/reperfusion in the hyperglycemic rat

作　　者：魏京娜 王晴川[2] 刘广芬[2] Edward L.Ezeli Michael J Quast 

机构地区：[1]Texas大学医学分校海洋生物医学研究所神经科学和细胞生物学系 [2]福建医科大学蛇毒研究所,福州350004 [3]Texas大学医学分校人类生化和基因西利结构生物学研究中心

出　　处：《海峡药学》2009年第12期8-15,共8页Strait Pharmaceutical Journal

摘　　要：目的应用核磁共振成像(MRI)技术,观察蕲蛇酶(Acutobin)对高血糖大鼠脑损伤体积和灌注不足的影响。方法在高血糖大鼠右脑中动脉阻塞/再灌注(MCAO/R)的动物模型上,在MCAO60min和再灌注后24h各静注2.5U.kg-1体重蕲蛇酶1次,在MCAO/R前和后,连续检测记录多断层扩散加权成像(multslice diffusion weighted imaging,DWI)和单一断层动态快速注射轨迹梯度回波成像(single slice dynamic bolus tracking gradien echo imaging,GE)。结果蕲蛇酶治疗组DWI所检测的损伤体积,在再灌注后明显减少(P<0.05),右脑半球脑血流残留量(residual CBF)明显增多,动物死亡率明显降低。结论蕲蛇酶能改善高血糖大鼠MCAO/R模型脑组织的灌流,减少梗塞面积,降低死亡率。OBJECTIVE Using the magnetic resonance imaging （MRI） technique to observe the effect of acu- tobin on MRI-detected brain lesion volume and tissue perfusion deficit in a hyperglycemic rat right middle cerebral artery occlusion/reperfusion （MCAO/R） model. METHODS of 2.5 u· kg^-bbodyweight 30 min afier MCAO and 24 h after （DWI） and single-slice dynamic bolus tracking gradient echo （ Acutobin was intravenously injected with a dosage reperfusion. Multislice diffusion weighted imaging GE） imaging were sequentially acquired before and after MCAO/R. RESULTS DWI-detected lesion volume was significantly （P 〈 0.05） reduced residual cerebral blood flow （CBF） in the right hemisphere recovered was significantly increased in acutobin group, and the mortality at 1 week after MCAO/R in the acutobin-treated group was significantly lower than the saline control group. CONCLUSION This indicate that acutobin improves brain tissue perfusion and reduces infarct volume and mortality in the hyperglycemic rat MCAO/ R model.

关 键 词：脑缺血 蕲蛇酶 溶栓性 去纤剂 核磁共振成像 高血糖 

分 类 号：R969[医药卫生—药理学]