高效液相色谱法测定雷公藤片中雷公藤甲素含量的方法改进  

Content determination of triptolide in TWH F. Tablets with Improved HPLC method

作　　者：黄秀旺[1] 许建华[1] 陈元仲 温彩霞[1] 吴国华[1] 

机构地区：[1]福建医科大学药学院临床药理研究所,福州350004 [2]福建省血液病研究所,福州350001

出　　处：《海峡药学》2009年第12期57-59,共3页Strait Pharmaceutical Journal

基　　金：福建省科技开发计划项目(2005D080);福建省重大专项前期研究项目(2005YZ1008)

摘　　要：目的开发出一种不用过氧化铝进行预处理柱的高效液相色谱法测定雷公藤片中雷公藤甲素含量的方法。方法雷公藤片粉末以无水乙醇超声溶解后直接上样测定。色谱柱为Gracesmart RP18柱(150×4.6mm,5μm),流动相为乙腈-水(21:79),流速1.0mL.min-1,柱温40℃,检测波长221nm,进样量5.0μL。结果雷公藤甲素检测浓度在0.5～3.0μg.mL-1范围内与峰面积积分值的线性关系良好(r=0.9995),平均加样回收率为96.25%(RSD=1.49%)。结论本方法简便、快捷、准确、重现性好,可用于雷公藤片的质量控制。OBJECTIVE To determine the content of triptolide in TWH F. tablets by HPLC with simple sample preperation. METHODS Samples were extracted with alcohol by ultrasound, the content determination was performed with acetonitrile-water（21：79） as mobil phase at a flow rate of 1.0mL·min^-1 and the detection wavelength at 221nm. RESULTS The linear detection concentration range of triptolide was 0.5-3.0μg· mL^- 1 （r = 0. 9995）, and the average recovery were 96.25 % （RSD = 1.49 % ）. CONCLUSION The method is simple, rapid, accurate and reproducible. It is adoptable for quantity analysis of triptolide in TWH F. tablets.

关 键 词：雷公藤甲素 雷公藤片 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]