HPLC法测定盐酸羟嗪含量及有关物质  

Determination of content and related substances of Hydroxyzine Hydrochloride by HPLC

作　　者：刘海燕[1] 崔艳[1] 严拯宇[1] 

机构地区：[1]中国药科大学分析化学教研室,南京211198

出　　处：《海峡药学》2009年第12期59-61,共3页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定盐酸羟嗪含量及有关物质的方法。方法采用C18柱,以磷酸氢二铵(0.01mol.L-1,pH6.0)-乙腈(60:40)为流动相,流速为1mL.min-1,检测波长为230nm,进样量20μL,柱温30℃。结果在浓度为5～50μg.mL-1范围内线性关系良好(r=0.9995,n=6),检测限为0.0083μg.mL-1。结论本法简便、准确、灵敏度高、重现性好,可作为盐酸羟嗪含量及有关物质的测定方法。OBJECTIVE To establish a HPLC method for the determination of the content and related sub- stances of Hydroxyzine Hydroehloride. METHODS The HPLC system is consisted of C18 column, the mobie phase is Ammonium hydrogen phosphate（0.01tool· L^- 1, pH6.0） ： acetonitrile（60 ： 40）. the flow rate was 1.0mL· min^- 1. Detection wavelength was set at 230nm, the injection volume was 20μL and column temperature was 30℃ RESULTS The calibration curve was linear in the range of 5.01 -- 50.10μg·mL^- l r = 0. 9995 （n = 6） ; the limit of detection was 0. 0.083μg·mL^- 1. CONCLUSION The method was rapid, simple, accurate sensitive and suitable for the content and related substances determination of Hydroxyzine Hydrochloride.

关 键 词：盐酸羟嗪 含量测定 有关物质 HPLC法 

分 类 号：R927.2[医药卫生—药学]