6种茎藤类药材同工酶的电泳分析  

Analysis of Isoenzymes in Six Kinds of Caulis Drug by Means of Polyacrylamide Gel Slab Electrophoresis

作　　者：赵华英[1] 陈永林[1] 霍德兰 陈沪宁[1] 

机构地区：[1]山东医科大学药学院

出　　处：《中国中药杂志》1998年第9期521-521,530,共2页China Journal of Chinese Materia Medica

摘　　要：目的：探索电泳法在鉴别茎藤类药材中的应用。方法：对６种性状较相似的茎藤类药材进行了过氧化物酶、酯酶和淀粉酶同工酶的电泳分析。结果：过氧化物酶同工酶的谱带最清晰，效果最理想，其谱带数及迁移率各不相同，能显示种间的明显区别。结论：过氧化物酶同工酶的电泳分析是鉴别茎藤类药材行之有效的方法之一。Objective:To probe into the possibility of application of electrophoresis in the identification of caulis drugs.Method:The isoenzymes of peroxidase,esterase and amylase in six kinds of caulis drug with similar characteristics were analyzed by means of polyacrylamide gel slab electrophoresis.Result:The bands of isoenzymogram of peroxidase are the clearest of all,and band number and mobility rates of the six drugs differ from one another,showing a remarkable interspecific distinction.Conclusion:Electrophoresis of isoenzymes of peroxidase can be employed as one of the effective means in defferentiating caulis drugs.

关 键 词：茎藤类 过氧化物酶 同工酶 电泳 中药鉴别 

分 类 号：R282.710.3[医药卫生—中药学]