杏花雨注射液对小鼠抗肿瘤作用的研究  

Study of the anti-tumor effect of Xinghuayu injection in mice

作　　者：牛培[1] 徐月清[1] 巴伟松[2] 李娟[3] 王晶 杜宁[5] 王乾[1] 

机构地区：[1]河北大学基础医学院,河北保定071000 [2]定州市人民医院,河北定州071012 [3]解放军252医院,河北保定071000 [4]北京万全阳光医药科技有限公司,北京100038 [5]保定市妇幼保健院,河北保定071000

出　　处：《医学研究与教育》2009年第6期11-14,共4页Medical Research and Education

基　　金：河北省科技厅项目(编号:062761399)

摘　　要：目的观察杏花雨注射液对肝癌(H22)模型小鼠的抑瘤作用。方法(1)抑瘤作用的研究:取昆明种小鼠,随机分为杏花雨17.5 mg/kg、35 mg/kg、70 mg/kg剂量组,以及环磷酰胺(20 mg/kg)对照组及模型组,每组小鼠均右前肢腋窝下接种肝癌H22瘤细胞。接种24 h后给药,每日1次。连续7 d,于末次给药24 h后,处死小鼠并剥出瘤块称重,计算抑瘤率。(2)增效作用的研究:取昆明种小鼠40只,随机分为模型对照组、杏花雨17.5 mg/kg剂量组、环磷酰胺(5 mg/kg)组、二药物合用组(杏花雨17.5 mg/kg、环磷酰胺5 mg/kg),每组小鼠均采右前肢腋窝接种瘤。24 h后给药,每天1次,连续7 d。于末次给药后24 h,处死小鼠,剥出瘤块称重,计算抑瘤率。结果(1)抑瘤作用:杏花雨注射液可以显著的抑制肝癌H22模型小鼠肿瘤的生长,17.5 mg/kg、35 mg/kg、70 mg/kg剂量组的抑瘤率分别为22.60%、48.84%、40.22%(P<0.01),环磷酰胺的抑瘤率为57.12%(P<0.01)。(2)增效作用:杏花雨注射液17.5 mg/kg剂量组、环磷酰胺(5 mg/kg)组、以及二药合用组抑瘤率分别为18.07%、23.88%、34.59%(P<0.01)。结论杏花雨注射液单独用药能够明显抑制荷瘤肿瘤细胞生长;与环磷酰胺合用能明显提高抑瘤效果。Objective To explore the anti-tumor effect of Xinghuayu injection in liver cance（rH22）model mice.Methods（1）Study on the anti-tumor effect： Kunming mice were divided into three groups： Xinghuayu injec-tion dose group,cyclophosphamide control group with the dose of 20mg/kg and model group.Doses of 17.5mg/ kg,35mg/kg,70mg/kg were administered to the Xinghuayu injection group,every mouse was inoculated tumor cell under the right forelimb axillary.The administration was started at 24 hours post inoculation and it was made once a day,which lasted 7 days.At 24 hours after the last administration,the mice was sacrificed and the tumor mass was unhooked to weight,then the tumor inhibitory rate was calculated.（2）Study on Synergism： 40 Kunming mice were randomly divided into model group,xinghuayu injection group with the dose of 17.5mg/kg,cyclophosphamide group with the dose of 5mg/kg,the combination group（xinghuayu 17.5mg/kg and cyclophos-phamide 5mg/kg）,every mouse was inoculated tumor cell under the right forelimb axillary.The administration was started at 24 hours post inoculation and it was made once a day,which lasted 7 days.At 24 hours after the last administration,the mice was sacrificed and the tumor mass was unhooked to weight.Results（1）anti-tu-mor effects： the Xinghuayu injection could significantly inhibit the growth of the liver cancer（H22）in model mice,the tumor inhibitory rate of the three groups,in which the dose respectively were 17.5mg/kg,35mg/kg,70mg/kg,were22.60%,48.84%,40.22%（P 0.01）,the tumor inhibitory rate of the cyclophosphamide was 57.12%（P0.01）.（2）The synergism： the inhibitory tumor rate of the xinghuayu group（17.5mg/kg）,cyclophos-phamide group（5mg/kg）and the combination group respectively were 18.07%,23.88%,34.59%（P0.01）.Conclusion Xinghuayu injection can significantly inhibit the growth of the tumor cells;using xinghuayu injec-tion with cyclophosphamide can significantly improve the killing tumor effects.

关 键 词：杏花雨注射液 抗肿瘤作用 抑瘤率 增效 

分 类 号：R979.1[医药卫生—药品]