蘑菇圈上四孢蘑菇个体间遗传差异的ISSR分析  

Genetic Relationships Between Agaricus campestris Fruit Bodies from Fairy Rings Based on ISSR Markers

作　　者：王波[1] 王一[1,2] 卢冰[1,2] 贾定洪[1] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066 [2]四川农业大学资源环境学院,四川雅安625014

出　　处：《食用菌学报》2009年第4期1-4,共4页Acta Edulis Fungi

基　　金：国家自然科学基金项目(编号:30771503);农业部948项目[编号:2006-G11(A)-01];农业部公益性行业(农业)科研专项(编号:nyhyzx07-008);四川省科技厅科技攻关项目(编号:05NG001-016-2)的部分研究内容

摘　　要：对四孢蘑菇（Agaricus campestris L.：Fr.）形成的蘑菇圈形态和子实体发生进行了调查,并对蘑菇圈上的四孢蘑菇个体间遗传差异进行了ISSR分析,建立遗传关系聚类图。结果表明,在生长于四川西部平坦高原草地上,四孢蘑菇形成的蘑菇圈为圆环状,蘑菇圈上生长的植物明显较蘑菇圈内、外生长的植物茂盛,同一蘑菇圈上生长的子实体生长期不完全一致;同一蘑菇圈上的四孢蘑菇个体之间相似系数为0.473～0.755,不同蘑菇圈上的个体遗传相似系数为0.333～0.604;在相似系数0.524水平上,不同蘑菇圈上的四孢蘑菇个体分别聚为不同类群。The geometrical configurations of annular fairy rings formed by the growth of Agaricus campestris fruit bodies were investigated,and the genetic relationships between nine fruit bodies from within a single ring and one fruit body from each of other three rings were analyzed using ISSR markers.Grass growing at the ring circumference was more abundant compared with that growing within and outside the circumference,while fruit bodies at different stages of development were observed in the same fairy ring.Genetic similarities based on ISSR markers revealed similarity coefficients ranging between 0. 473～0. 755 and 0. 333～0. 604 for fruit bodies from the same fairy ring and from different fairy rings, respectively. A dendrogram constructed using the ISSR amplification data separated mycelia isolated from 12 fruit bodies growing in four different fairy rings into four groups at a similarity coefficient of 0. 524.

关 键 词：四孢蘑菇 蘑菇圈 子实体 遗传差异 ISSR 

分 类 号：S646.11[农业科学—蔬菜学]