不同切肝量的大鼠血清HSP70变化及其意义  

Variation and significance of serum HSP70 in different hepatectomy mice

作　　者：黄建康[1] 卢榜裕[1] 孙志刚[1] 荀敏[1] 李建军[1] 晏益核[1] 肖顺崇[1] 黄琴陶[1] 

机构地区：[1]广西医科大学第一附属医院微创外科中心,广西南宁530007

出　　处：《中国现代医学杂志》2009年第24期3708-3712,3717,共6页China Journal of Modern Medicine

摘　　要：目的探讨大鼠血清HSP70在评价手术创伤程度中的意义。方法建立大鼠不同切肝量的手术创伤模型,310只雄性SD大鼠随机分为正常组、轻度切肝创伤组、中度切肝创伤组、重度切肝创伤组及谷氨酰胺干预组,并予术后2、8、12、24和48h检测大鼠血清中HSP70、皮质醇的浓度和肝功能的变化。结果手术创伤后2h血清HSP70表达开始增加,12h达到高峰,48h仍高于正常水平。血清皮质醇于术后2h迅速增加,8h达到高峰,24h仍有较高浓度,48h略高于正常。谷氨酰胺干预后,HSP70在各时相点均增高,皮质醇浓度均有所下降。中、重度切肝手术创伤组术后的ALT、TB均出现异常,经谷氨酰胺干预后两指标均有所改善。结论手术创伤可以引起血清HSP70和皮质醇浓度的明显升高,随着创伤程度的增加而显著升高。血清中检测HSP70的表达可以作为判断手术创伤程度的指标。术前诱导HSP70的高表达有利于增强对机体的保护作用。【Objective】To discuss the significance of serum HSP70 in the evaluation of degree of surgical trauma.【Methods】To establish surgical trauma models of rats about different hepatectomy.A total of 310 rats were randomly divided into control group,mild hepatic trauma group,moderate hepatic trauma and severe hepatic trauma group and Glutamine intervention group;the expression of HSP70,corticosterone levels and liver function in serum were detected in 2,8,12,24 and 48 h after hepatectomy respectively.【Results】The HSP70 expression in serum increased at 2 h after the surgical trauma,12 h peaked,48 h at a high level.Serum corticosterone increased rapidly at 2 h after operation,8 h peak,48 h slightly higher than normal.After Glutamine intervention,the HSP70 expression increased while serum corticosterone declined at various time points.ALT and TB were significantly higher than the normal range among moderate and severe hepatic trauma group after operation;the indicators of ALT and TB were improved after the intervention of glutamine.【Conclusion】Serum HSP70 and corticosterone concentration are markedly elevated after surgical trauma,serum HSP70 expression can be used as indicator to diagnose the degree of surgical trauma.Preoperative induction of HSP70 expression has the protective effect for the body.

关 键 词：热休克蛋白70 皮质醇 肝切除术 谷氨酰胺 

分 类 号：R456.4[医药卫生—治疗学]