牛睾丸差异表达基因Septin10的筛选、鉴定及组织表达研究  

Screening,Identification and Tissue Expression Analysis of Septin 10 Gene Differentially Expressed in the Testis of Crossbred Bulls

作　　者：李姣[1] 陈若愚[1] 高雪[1] 李俊雅[1] 张路培[1] 周正奎[1] 陈金宝[1] 许尚忠[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所中国农业科学院肉牛研究中心农业部畜禽遗传资源与利用重点开放实验室,北京100193

出　　处：《畜牧兽医学报》2010年第1期27-32,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十一五"国家高技术研究计划(863计划)(2008AA10Z146);国家科技支撑计划(2006BAD014A10;2006BAD04A16)

摘　　要：本研究旨在筛选出决定或影响公牛睾丸周长(SC)的基因片段,并研究其功能和表达特征,为今后种公牛的选育提供理论基础。用mRNA差异显示技术从睾丸周长极显著差异(P<0.01)的睾丸组织中筛选差异表达的基因片段,并用反Northern杂交及荧光定量的方法鉴定筛选的结果,研究筛选出基因的组织表达谱。结果,筛选出1条差异表达片段A71,经测序和BLAST分析发现该片段与牛Septin10基因高度同源(99%),且该基因在肝脏等9个组织中都有表达,在2组睾丸组织中相对表达量差异显著(P<0.05)。分析结果表明,Septin10基因是牛睾丸差异表达基因,可作为一个研究牛繁殖性状的候选基因。This study was designed to detect differential gene expression fragments in bulls of two groups which had significant difference in scrotal circumference（SC）（P〈0.01）,and analyze their expression characteristics.The differential gene expression fragments were detected by differential display RT-PCR,then were identified by the reverse Northern blot analysis and real-time PCR analysis,finally the expression profile of the differential gene was analyzed.One specific fragment named A71 was found.After sequencing and BLAST analysis,A71 showed high homology（99%） to bovine Septin 10 gene.The gene was expressed in various tissues,and showed significant difference between the two groups（P〈0.05）.The results indicated that the Septin 10 gene was differential expression gene in testicle of bulls and it might be a candidate gene in regulating reproduction traits in bulls.

关 键 词：睾丸周长 DDRT-PCR Septin10 反Northern杂交 荧光定量PCR 组织表达谱 

分 类 号：S823[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]