α-酮戊二酸对LPS慢性应激仔猪小肠黏膜形态与功能的影响  

The Effects of α-Ketoglutarate on Intestinal Mucosal Morphology and Function in Piglets Chronically Challenged with Lipopolysaccharide

作　　者：王蕾[1] 刘坚[1] 侯永清[1] 丁斌鹰[1] 刘玉兰[1] 朱惠玲[1] 李永塘[1] 吴信[2] 

机构地区：[1]武汉工业学院动物营养与饲料科学湖北省重点实验室,武汉430023 [2]中国科学院亚热带农业生态研究所动物营养代谢过程与生理调控试验室,长沙410125

出　　处：《畜牧兽医学报》2010年第1期46-52,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30871801);湖北省自然科学基金计划创新群体项目(2007ABC009);湖北省高等学校优秀中青年团队计划项目(T200810)

摘　　要：为了探讨α-酮戊二酸(AKG)能否缓解LPS慢性应激导致的仔猪小肠黏膜损伤及其机理,本试验研究了AKG对LPS慢性应激仔猪的小肠黏膜形态、血浆D-木糖的含量、血浆和小肠黏膜二胺氧化酶(DAO)活性及小肠黏膜mTOR及磷酸化的mTOR表达量的影响。18头(24±1)日龄健康断奶仔猪随机分成3个处理组(空白对照组、应激对照组、AKG组),每个处理6个重复。各组基础日粮一致,空白对照组和应激对照组饲喂基础日粮+1%淀粉,AKG组饲喂基础日粮+1%AKG。试验期为16 d。应激对照组和AKG组仔猪分别于第10、12、14和16天腹膜注射80μg.kg-1BW的LPS,空白对照组注射相应剂量的灭菌生理盐水。第16天注射LPS 2 h后,按0.1g.kg-1BW的剂量给仔猪灌服D-木糖溶液,注射LPS 3 h后,前腔静脉采血。第17天屠宰取小肠组织样,刮取肠黏膜及制作组织切片。结果表明:(1)与空白对照组相比,应激对照组十二指肠、空肠和回肠黏膜绒毛高度/隐窝深度、空肠和回肠磷酸化mTOR/mTOR(P-mTOR/mTOR)显著降低(P<0.05),血浆DAO活性显著升高(P<0.05)。(2)与应激对照组相比,AKG组十二指肠、空肠和回肠黏膜绒毛高度/隐窝深度、空肠黏膜DAO活性、血浆D-木糖及十二指肠、空肠和回肠黏膜P-mTOR/mTOR显著升高(P<0.05)。结果显示,日粮中添加1%AKG可在一定程度上改善仔猪的小肠组织学形态和吸收功能,缓解LPS慢性应激导致的仔猪小肠黏膜损伤,这与mTOR信号通路有关。This study was conducted to investigate the effects of α-ketoglutarate（AKG） on small intestinal morphology,plasma D-xylose concentration,activities of diamine oxidase（DAO） in plasma and intestinal mucosa,and expression of mammalian target of rapamycin（mTOR） with phosphorylation（P-mTOR/mTOR） in the piglets under lipopolysaccharide（LPS） challenge.Eighteen healthy crossbred（Duroc×Landrace×Yorkshire） piglets were randomly assigned into 3 treatment groups（6 replicates per group）： control group,LPS group and AKG group.The control and LPS groups were fed the basal diet＋1% starch,and the AKG group was fed the basal diet＋1% AKG.On d 10,12,14 and 16,the piglets in the LPS and AKG groups were injected intraperitoneally with LPS at 80 μg·kg-1BW,whereas piglets in the control group were injected intraperitoneally with the same dose of physiological saline.On d 16,D-xylose was orally administrated to all pigs at the dose of 0.1g·kg-1BW 2 h after LPS challenge,and blood samples were collected 3 h after LPS challenge.All the piglets were sacrificed on d 17 to examine small intestinal morphology and collect intestinal mucosa for analysis.The results showed that：（1） Compared to the control group,the ratio of villus height to crypt depth in duodenum,jejunum and ileum,P-mTOR/mTOR in jejunal and ileal mucosa of LPS group significantly decreased（P〈0.05）,while the activities of DAO in plasma significantly increased（P〈0.05）.（2） Compared to the LPS group,the ratio of villus height to crypt depth in the duodenum and jejunum ileum,activities of DAO in the jejunal mucosa,D-xylose content in plasma and P-mTOR/mTOR in duodenal,jejunal and ileal mucosa of AKG group significantly increased（P〈0.05）.In conclusion,dietary supplementation with 1% AKG could improve small intestinal morphology and Absorption function to some extent and alleviate the intestinal mucosal damage caused by LPS challenge in piglets,which were associated with mTOR signaling pathway.

关 键 词：Α-酮戊二酸 仔猪 小肠黏膜 MTOR信号通路 脂多糖 

分 类 号：S828[农业科学—畜牧学] S816.7[农业科学—畜牧兽医]