应用重组肌肉抑制素C-端重组蛋白提高仔鼠出生质量的研究  

Immunization of Mother Mice with Recombinant Myostatin C-domain Increasing the Body Weight of Neonatal Mice

作　　者：马毅[1] 李晴[2] 陈小强[3] 王文杰[1] 关宏[2] 安晓荣[2] 陈永福[2] 

机构地区：[1]天津市畜牧兽医研究所,天津300112 [2]中国农业大学农业生物技术国家重点实验室,北京100193 [3]天津农学院农学系,天津300384

出　　处：《畜牧兽医学报》2010年第1期112-116,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：天津市自然科学基金项目资助(08JCYBJC04500);国家转基因生物新品种培育重大专项(2008zx08008-005)

摘　　要：为探讨利用重组肌肉抑制素促进肌肉生长的可能性,本研究原核表达并纯化了肌肉抑制素C-端重组蛋白。将雌性小鼠同雄性小鼠合笼,妊娠后分笼饲养。应用重组蛋白免疫雌性昆明白小鼠。利用间接ELISA法检测抗血清效价。每周测母鼠及仔鼠体质量1次。结果表明:(1)免疫后对照组和试验组母鼠体质量差异不显著(P>0.05)。在妊娠后期(免疫后第11周),两组间体质量出现差异(P<0.05),试验组为(56.23±3.37)g,而对照组为(54.11±3.18)g。试验组体质量在第12周达到(79.16±3.72)g,而对照组为(75.23±3.44)g,差异极显著(P<0.01)。分娩后,这种差异消失(P>0.05)。(2)仔鼠出生体质量在2处理组间差异极显著(P<0.01),试验组仔鼠出生体质量比对照组增加25.8%左右;但在生长阶段仔鼠体质量差异有逐渐缩小的趋势。结论:应用肌肉抑制素C-端重组蛋白免疫母鼠可以显著提高仔鼠出生体质量。The potential of using recombinant MSTN as vaccine to improve muscle mass was explored in this study.Firstly,the recombinant myostatin C-domain was obtained through prokaryotic expression and purification.Then female Kunming White mice after immunization with recombinant C-domain were mated with male mice.The indirect ELISA assay was used to detect the titer of antibody against MSTN in mother mice.The body weights of mice were evaluated each week.The results showed：（1） The body weight of mother mice in experiment group didn′t change significantly compared with that in control group（P〉0.05）.But during the late pregnancy（the 11th week after immunization）,the increase of body weight of experiment group exceeded significantly that in control group（P〈0.05）.The body weight was（56.23±3.37）g in experiment group,and（54.11±3.18）g in control group.In the 12th week after immunization,the body weight of experiment group was（79.16±3.72）g,and that of control group was（75.23±3.44）g.The former was significantly higher than the latter（P〈0.01）.After the delivery,the difference of body weight in two groups disappeared again（P〉0.05）;（2） The body weight of neonatal mice in experiment group was 25.8% higher than that in control group（P〈0.01）,but this difference decreased with the development of neonatal mice.These results proved that immunization of mother mice with recombinant MSTN C-domain protein could significantly increase the body weight of neonatal mice.

关 键 词：肌肉抑制素 免疫 小鼠 抗体 体质量 

分 类 号：Q784[生物学—分子生物学]