EphA3受体截短突变体variant b在前列腺癌细胞中的分泌表达  

Expression and Secretion Characteristic of EphA3 Variant b in Prostate Cancer Cell Lines

作　　者：钱晓龙[1] 庞博[1] 施庆国[1] 武瑞琴[1] 俞岚[1] 李山虎[1] 周建光[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2010年第1期27-31,共5页Letters in Biotechnology

基　　金：国家自然科学基金(30770834;30870961);国家高技术研究发展计划(2008AA02Z123)

摘　　要：目的:验证EphA3受体截短突变体variant b是否是一种分泌蛋白,并探索其内源分泌的特点,在细胞水平看其是否有作为前列腺癌血清标志物的潜质。方法:构建EphA3 variant b真核表达载体,应用标签抗体、特异性抗体和Western印迹检测前列腺癌细胞培养液中EphA3 variant b的表达,用RT-PCR方法分析EphA3 variant b在8种细胞系中的表达谱和对雄激素刺激的应答。结果:验证了EphA3 variant b是一种分泌蛋白,在雄激素受体阳性的前列腺癌细胞系中特异性表达,雄激素以剂量依赖方式诱导EphA3 variant b表达。结论:EphA3 variant b是一种分泌蛋白,其表达与雄激素受体信号通路相关,有作为前列腺癌血清标志物的潜质。Objective：To validate the truncated mutant of EphA3 receptor（EphA3 variant b）a secretary protein and to explore its endogenous secretion character,as well as to test if it can be used as a serum biomarker for prostate cancer on cellular level.Methods：By constructing eukaryotic expression vector and Western blotting with anti-His tag antibody as well as specific antibody,EphA3 variant b was tested if it can be expressed in cell culture fluid of prostate cancer cell lines.Then the expression spectrum of EphA3 variant b in eight cell lines as well as its response to androgen stimulus in LNCaP was analyzed by RT-PCR.Results：EphA3 variant b is a kind of secretary protein,which is specifically expressed in AR＋prostate cancer cell lines and dose-dependently induced by androgen.Conclusion：EphA3 variant b is a kind of secretary protein,which is androgen-receptor signal pathway associated and has its potential as a serum biomarker for prostate cancer.

关 键 词：前列腺癌 血清标志物 分泌蛋白 EphA3截短突变体variant b 

分 类 号：Q579.11[生物学—生物化学] TQ245.23[化学工程—有机化工]