亚砷酸和顺铂对骨肉瘤MG-63细胞生长的抑制作用比较  

Comparison of arsenic trioxide and cisplatin on inhibiting osteosarcoma MG-63 cells

作　　者：李雪松[1] 刘贾昆[1] 王文波[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,150001

出　　处：《中国地方病学杂志》2010年第1期37-41,共5页Chinese Jouranl of Endemiology

基　　金：基金项目：黑龙江省教育厅科学技术研究项目（11531099）;黑龙江省卫生厅科研课题资助项目（2007-190）;黑龙江省中医药局、中西医结合重点科研基金（2009-2011）

摘　　要：目的观察亚砷酸和顺铂对骨肉瘤MG-63细胞生长的抑制作用。方法采用细胞培养的方法，体外培养人骨肉瘤株MG-63细胞。应用倒置相差显微镜、透射电镜、四氮唑盐（MTT）比色、流式细胞仪检测等方法，观察亚砷酸和顺铂[各加入100μl（1g/L）]对骨肉瘤MG-63细胞生长的抑制作用和对细胞凋亡、细胞周期的影响。结果相差显微镜下，对照组MG-63细胞贴壁生长良好，细胞核呈圆形，细胞呈上皮样细胞排列：亚砷酸组MG-63细胞排列无规则，有细胞死亡；顺铂组MG-63细胞多变小，变圆，胞质的空泡化明显。电镜下，对照组MG-63细胞表面可见球形质膜突起，线粒体嵴粗大，双层核膜结构清晰：亚砷酸组MG-63细胞表面的球形突起消失，线粒体嵴变细，细胞核缩小，凋亡细胞核膜清晰；顺铂组MG-63细胞表面球形突起脱离。细胞核膜增厚，核内染色质形成细纱状。亚砷酸和顺铂均能抑制MG-63细胞生长，抑制率（％）组间比较差异有统计学意义（F=78．859，P〈0．05）；在2、4、8、16、32、64、128h，亚砷酸（56．31±0．03、70．00±0．06、79．84±0．03、87．31±0．13、84．70±0．09、90．68±0．06、91．18±0．05）和顺铂（7．55±0．15、15．70±0．17、30．72±0．07、49．80±O．05、45．11±0．13、61．62±0．08、93．80±0．12）对细胞生长的抑制率与对照组（2．03±0．07、2．78±0．08、3．11±0．01、5．67±0．04、12．23±0．04、18．65±0．04、24．45±0．04）比较明显升高（P均〈0．05）；但与顺铂比较，亚砷酸起效快，在2～32h抑制率明显高于同时间顺铂（P均〈0．05）。亚砷酸和顺铂均可导致MG-63细胞凋亡，凋亡率组间比较差异有统计学意义（F=13．317，P〈0．05）；亚砷酸在24、36、48h（20．50±3．78、45．76±9．90、25．16±15．41），顺铂在24、36、48h（12．55±1．51、18．85±3．40、12．37±5．43），MG-63�Objective To explore the inhibiting effects of arsenic trioxide and cisplatin on MG-63 cells. Methods Using MTT assay, flowcytometry, phase contrast microscopy and electron microscopy methods, the therapeutic effect of arsenic trioxide was studied for the osteosarcoma in the cultured MG-63 cells in vitro, and compared these effects with cisplatin. The inhibitory rates of cell growth and the effect of apoptosis and cell cycle were compared between arsenic trioxide and cisplatin on MG-63 cells. Results The contrast phase microscope revealed the adhesion ability of normal groups was good and cellular morphology showed epithelium ceils. But the cellular morphology showed irregular arrangement in arsenic trioxide groups and cytoplasmic vacuoles in cisplatin group. Electron microscope revealed the globular plasmalemma ecphymas in cell surface of control groups, theenlarged crista mitochondriales and the double-deck membrane structure appeared clearly. But electron microscope revealed globular plasmalemma processes in cell surface of arsenic trioxide groups, thinned crista mitochondriales and clearly seen karyopycnosis and nuclear membrane of apoptotic cells. The globular plasmalemma processes in cell surface of cisplatin groups were separated, nuclear membrane thickened and chromatin were in sandy shape. Both arsenic trioxide and cisplatin inhibited effectively MG-63 cells growth. There was a significant difference in different groups of inhibition ratios to the growth of cells（all P 〈 0. 05）. In 2,4,8,16,32,64,128 hours, the inhibition ratios（%） of arsenic trioxide（56.31 ± 0.03,70.00 ± 0.06,79.84 ± 0.03,87.31 ± 0.13,84.70 ± 0.09, 90.68 ± 0.06,91.18 ± 0.05） and cisplatin groups（7.55 ± 0.15,15.70 ± 0.17,30.72 ± 0.07,49.80 ± 0.05,45.11 ± 0.13,61.62 ± 0.08,93.80 ± 0.12） were obviously increased as compared with those in the control group（2.03 ± 0.07,2.78 ± 0.08,3.11 ± 0.01,5.67 ± 0.04,12.23 ± 0.04,18.65 ± 0.04,24.45 ± 0.04, all P 〈 0.05）. Moreover the inhibition ratio of arseni

关 键 词：亚砷酸 顺铂 MG-63细胞 细胞凋亡 细胞周期 

分 类 号：R686[医药卫生—骨科学]