绿脓杆菌及其鞭毛蛋白联合rhIL-12体外对慢性乙型肝炎患者细胞免疫功能的影响  

In Vitro Effect of Piliated Pseudomonas aeruginosa and Its Flagellin Combined with rhIL-12 on Immunological Function of Patients with Chronic Hepatitis B

作　　者：傅泳航[1] 杨向阳[2] 李茹冰[3] 王增松 王翠玲 谢飞群 牛丽红 邓凤君[3] 徐江平[3] 张宜俊 

机构地区：[1]解放军第458医院检验科,广州510602 [2]广州军区机关门诊部保健科,广州510080 [3]南方医科大学药学院,广州510515 [4]广州市恺泰生物科技有限公司,广州510620

出　　处：《中国生物制品学杂志》2010年第1期39-42,共4页Chinese Journal of Biologicals

基　　金：广东省科技计划项目基金(2007B060401053);广州经济技术开发区科技发展资金(2008Q-P064)

摘　　要：目的探讨绿脓杆菌及其鞭毛蛋白联合rhIL-12体外对慢性乙型肝炎患者细胞免疫功能的影响,为rhIL-12作为佐剂应用于临床提供理论依据。方法取健康人及慢性乙型肝炎患者外周血单个核细胞(PBMCs),分别与绿脓杆菌、鞭毛蛋白、rhIL-12、绿脓杆菌+rhIL-12、鞭毛蛋白+rhIL-12孵育,ELISA法检测细胞培养上清中IFNγ的含量。结果绿脓杆菌和鞭毛蛋白组只诱导产生低水平的IFNγ,rhIL-12组诱导产生IFNγ的水平较阴性对照组显著提高;而绿脓杆菌+rhIL-12组、鞭毛蛋白+rhIL-12组诱导产生IFNγ的水平显著高于绿脓杆菌、鞭毛蛋白和rhIL-12组,差异均有统计学意义,且rhIL-12的作用呈剂量依赖性。结论绿脓杆菌及其鞭毛蛋白联合rhIL-12体外能显著提高健康人和慢性乙型肝炎患者PBMCs产生IFNγ的水平,增强其细胞免疫应答。Objective To investigate the in vitro effect of piliated Pseudomonas aeruginosa（PPA）and its flagellin combined with rhIL-12 on the immunological function of patients with chronic hepatitis B （HB）.Methods The PBMCs of patients with chronic HB were co-incubated with PPA,the flagellin of PPA,rhIL-12,PPA ＋ rhIL-12 and flagellin ＋ rhIL-12 respectively,using those of healthy persons as control.The IFNγ content in culture supernatant of PBMCs was determined by ELISA.Results PPA and its flagellin induced only low IFNγ levels,while the IFNγ level induced by rhIL-12 was significantly higher than that of negative control.However,the IFNγ levels induced by PPA ＋ rhIL-12 and flagellin ＋ rhIL-12 were significantly higher than those by PPA,flagellin and rhIL-12,and the effect of rhIL-12 was dose-dependent.Conclusion PPA and its flagellin combined with rhIL-12 significantly increased the IFNγ levels in PBMCs of patients with HB and healthy persons in vitro,and enhanced their cellular immune response. 【Key Words】： Piliated Pseudomonas aeruginosa（PPA） Flagellin IL-12 PBMCs IFNγ Chronic hepatitis B

关 键 词：绿脓杆菌制剂 鞭毛蛋白 白细胞介素-12 外周血单个核细胞 干扰素Γ 慢性乙型肝炎 

分 类 号：R512.62[医药卫生—内科学]