重组弓形虫Peroxiredoxin蛋白诱导小鼠的免疫应答及其抗弓形虫感染的保护作用  

Induction of Immune Response and Protection against Toxoplasma gondii Infection by Recombinant T.gondii Peroxiredoxin Protein in Mice

作　　者：吴锴[1,2] 胡建敏[1,2] 殷国荣[1] 曹蕾[1] 葛玉芳[1,2] 刘美娜[1] 孟晓丽[1] 

机构地区：[1]山西医科大学医学寄生虫学研究所寄生虫学教研室,太原030001 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国生物制品学杂志》2010年第1期51-53,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金(30640057);山西医科大学青年基金(02200713)

摘　　要：目的观察不同剂量重组弓形虫Peroxiredoxin蛋白(rTgPrx)诱导小鼠的体液免疫、细胞免疫应答及其抗弓形虫感染的保护作用。方法BALB/c小鼠60只,随机分为5组,分别用20、40、60、80μgrTgPrx(溶于100μlPBS中)和100μlPBS皮下免疫小鼠3次,间隔2周。末次免疫后第14天,用1×104个速殖子/只灌胃攻击,逐日观察小鼠健康状况。攻击后第30天,ELISA法检测小鼠血清IgG和小肠冲洗液sIgA水平,分离并计数脾淋巴细胞和小肠上皮内淋巴细胞(IEL),分离肝、脑组织内弓形虫速殖子并计数。结果60μgrTgPrx组小鼠血清IgG水平显著高于PBS、20μg和40μgrTgPrx组,小肠冲洗液sIgA组间比较差异无统计学意义;80μgrTgPrx组小鼠脾淋巴细胞数量明显高于PBS和20μgrTgPrx组,IEL数量各组间差异无统计学意义;60μg和80μgrTgPrx组小鼠脑和肝组织虫荷均低于PBS和20μgrTgPrx组。结论60μg和80μgrTgPrx皮下免疫小鼠可诱导有效的体液免疫、细胞免疫应答,且抗弓形虫感染的保护作用效果良好。Objective To observe the humoral and cellular immune responses as well as protection against Toxoplasma gondii infection induced by recombinant T.gondii peroxiredoxin protein （rTgPrxz）at various dosages in mice.Methods Sixty BALB /c mice were divided into five groups randomly,and injected s.c.with 20,40,60,80 μg of rTgPrx（dissolved in 100 μl of PBS）and 100 μl of PBS respectively for 3 times at intervals of 2 weeks.Each mouse was challenged with 1 × 10^4 tachyzoites by intragastric route on day 14 after the last immunization and observed for health daily.All the mice were killed on day 30 after challenge and determined for IgG in sera and sIgA in intestine washes.The splenic lymphocytes and intestinal intraepithelial lymphocytes （IELs）of mice were isolated and counted.Meanwhile,the tachyzoites in livers and brains of mice were counted.Results The serum IgG levels of mice injected with 60 μg of rTgPrx were significantly higher than those with 20 and 40 μg of rTgPrx and with PBS.However,no significant differences were observed in the sIgA levels in intestine washes of mice in various groups.The counts of splenic lymphocytes were significantly higher in 80 μg of rTgPrx group than in PBS and 20 μg of rTgPrx groups,while those of IELs showed no significant difference in various groups.The counts of tachyzoites in livers and brains of mice in 60 and 80 μg of rTgPrx groups were significantly lower than those in PBS and 20 μg of rTgPrx groups.Conclusion Immunization with 60 and 80 μg of rTgPrx by subcutaneous injection induced effective humoral and cellular immune responses as well as protection against Toxoplasma gondii infection in mice.

关 键 词：刚地弓形虫 PEROXIREDOXIN 重组蛋白 免疫应答 免疫保护 

分 类 号：R382.5[医药卫生—医学寄生虫学]