STR图谱分析法鉴别人源细胞交叉污染  

Identification of Cross Contamination of Human Cell Lines by STR Profiling

作　　者：吴瑜[1] 冯建平[1] 林林[1] 吴雪伶[1] 孟淑芳[1] 王佑春[1] 李德富[1] 

机构地区：[1]中国药品生物制品检定所细胞室卫生部国家生物技术产品质量标准化重点实验室,北京100050

出　　处：《中国生物制品学杂志》2010年第1期74-78,86,共6页Chinese Journal of Biologicals

基　　金：国家科技支撑计划"生物制品安全性评价技术及原辅料安全性研究--生物制品生产用细胞基质安全性研究"(2008BAI54B00-4)

摘　　要：目的采用STR图谱分析法鉴别人源细胞交叉污染。方法采用PCR-毛细管电泳分析方法,分别检测人源、猴源及鼠源细胞以及人源细胞交叉污染模型的16个STR位点,分析STR图谱法用于细胞交叉污染检测的可行性。并对来自不同生物制品生产单位的27株生产用细胞及5株组织工程医疗产品用细胞进行STR图谱分析。结果STR图谱分析方法具有人源细胞特异性,猴源细胞及鼠源细胞不产生特征性图谱。此方法可以对形态相似或不相似的人源细胞交叉污染模型进行明确鉴别。采用此方法检测的27株生物制品生产用人源细胞未发现有交叉污染现象,而5株组织工程医疗产品用细胞中,发现有2株为非单个个体的混合细胞。结论STR图谱分析法具有高特异性,可用于人源细胞间交叉污染或细胞个体来源的鉴别。Objective To identify the cross contamination of human cell lines by STR profiling.Methods The 16 STR loci of human,monkey and mouse cell lines as well as cross contamination model of human cell line were determined by PCR-capillary electrophoresis to evaluate the feasibility of the method to identification of cell cross contamination.Twenty-seven human cell lines used for production of biologics and 5 cell lines used for production of tissue engineering products,from various manufactures,were analyzed by the developed STR profiling method.Results Human cell lines showed characteristic STR profiles,while monkey and mouse cell lines showed no such profiles.The developed STR profiling method could be used for authentication of cross contamination models of human cell lines with morphological difference or similarity.No cross contamination was found in 27 human cell lines for production of biologics.However,2 cell lines used for production of tissue engineering products were identified as mixed cells.Conclusion STP profiling showed high specificity and could be used for authentication of cross contamination between human cell lines or origin of cell individual.

关 键 词：STR图谱 人源细胞 组织工程产品 交叉污染 

分 类 号：Q78[生物学—分子生物学] R311[医药卫生—基础医学]