复方活血汤对放射性损伤小鼠造血细胞bcl-2、bax、bcl-xL基因表达的作用  

Effect of compound blood activating decoction on expression of bcl 2,bax and bcl x L genes in bone marrow hematopoietic cells of radiation injured mice.

作　　者：刘文励[1] 孙汉英[1] 周剑锋[1] 肖侃艳[1] 唐锦治[1] 

机构地区：[1]同济医科大学附属同济医院内科

出　　处：《中华放射医学与防护杂志》1998年第4期256-258,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金

摘　　要：目的探讨放射性损伤骨髓衰竭的机理及防护对策。方法用Ｎｏｒｔｈｅｒｎｂｌｏｔ技术检测了６０Ｃｏγ射线８．０Ｇｙ照射小鼠（对照组）及照射后立即给复方活血汤治疗小鼠（中药组）的骨髓有核细胞ｂｃｌ－２、ｂａｘ、ｂｃｌ－ｘＬ基因表达。结果放射损伤后第３，７天，对照组骨髓有核细胞ｂｃｌ－２／ｂａｘ值减小、ｂｃｌ－ｘＬ不表达。中药组与之相反，ｂｃｌ－２／ｂａｘ值明显增大、ｂｃｌ－ｘＬ明显表达。受照后第７天两组脾脏单个核细胞ｂｃｌ－２／ｂａｘ值与骨髓有核细胞的表达一致。结论复方活血汤对放射损伤骨髓造血细胞增殖周期调控基因ｂｃｌ－２、ｂａｘ、ｂｃｌ－ｘＬ有一定调节作用。Objective\ To explore the mechanism of and protective strategy againt bone marrow failure caused by radiation injury.Method\ Northern blot technique was used to detect the bcl 2,bax and bcl x L gene expression in murine bone marrow nucleated cells (BMNC) both in Chinese drug treated group which was given per os compound blood activating decoction immediately after irradiation with 8.0 Gy60Co γ rays,and in control group.Results\ On the 3rd and 7th  day after irradiation,bcl 2/bax value of BMNC decreased obviously.No bcl x L expression was observed in control group.In Chinese drug treated group,the bcl 2/bax value increased remarkably,while bcl x L expression occurred obviously.On the 7th day after irradiation,the bcl 2/bax value in splenic mononuclear cells was consitstent with that in BMNC in both Chinese drug treated and control groups.Conclusion\ Compound blood activating decoction could have a regulative effect on the expression of cell cycle regulation gene bcl 2,bax and bcl xL in irradiated bone marrow hematopoietic cells and protect the bone marrow hematopoiesis.

关 键 词：放射损伤 造血细胞 基因表达 复方活血汤 

分 类 号：R818.74[医药卫生—放射医学] R289.5[医药卫生—临床医学]