利用肺癌特异性结合多肽ZS-6筛选肺癌相关标志物  

Screening of tumor mark for the lung cancer by using peptide ZS-6

作　　者：冯文彬[1] 潘雪刁[1] 周捷[1] 石磊[1] 罗军强[1] 李询[1] 臧林泉[1] 

机构地区：[1]广东药学院药科学院药理学教研室,广东广州510006

出　　处：《中国药理学通报》2010年第1期44-47,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30572177);教育部科学技术研究重点项目(No208105);广东药学院院内科研启动基金(No06);2008师资队伍建设专项经费资助

摘　　要：目的利用本实验室已获得的与肺癌特异性结合的十二肽ZS-6从人类肺癌cDNA文库中筛选出肺癌相关标志物,为肺癌的临床早期诊断和靶向治疗奠定基础。方法以生物素标记的十二肽ZS-6为探针,筛选人类肺癌cDNA噬菌体展示文库,经过四轮减性筛选后,挑取与ZS-6特异性结合的噬菌体单克隆、扩增、纯化,再通过PCR技术扩增、产物纯化后,进行DNA测序,测序结果经过生物信息学分析。结果筛选出18个与ZS-6特异性结合的噬菌体克隆,测序结果显示其中1个为未知功能的新基因,其余的均已确定为肿瘤相关基因。结论从人类肺癌cDNA噬菌体展示文库中筛选出潜在的肺癌肿瘤标志物,为肺癌的早期诊治奠定基础。Aim To select a potential biomarker for early lung cancer diagnosis and targeted therapy by using the cancer specific bounded peptide ZS-6 which had already been obtained from the laboratory. Methods The peptide ZS-6 marked by biotin was used as a probe to pan out the human lung cancer cDNA phage display library,after 4 rounds of subtraction panning, the specific binding clones of ZS-6 were identified. After amplification and purification,then those DNA sequences were identified and analyzed with bioinformat-ics. Results 18 phage clones were identified to the specific peptide ZS-6 and the DNA sequence showed one of them was an unknown new gene while the others were known tumor related genes. Conclusion A tumor biomarker selected from human lung cancer cDNA library provides a potential tool for early lung cancer diagnosis and therapy.

关 键 词：多肽ZS-6 CDNA文库 减性筛选 肺癌 肿瘤标志物 

分 类 号：R342.3[医药卫生—基础医学] R394.2