缓释骨形态发生蛋白-2听泡内植入对豚鼠内耳功能的影响  

The Affection on Cochlea Function of Guinea-pig by Transplanting Controlled Release BMP-2 into the Middle Ear

作　　者：李雪盛[1] 孙建军[1] 姜伟[1] 刘肖[2] 

机构地区：[1]海军总医院全军耳鼻咽喉科中心,北京100048 [2]海军总医院病理科

出　　处：《听力学及言语疾病杂志》2010年第1期39-42,共4页Journal of Audiology and Speech Pathology

基　　金：中国博士后科学基金项目(20080440224)

摘　　要：目的观察缓释骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)植入豚鼠听泡内的成骨诱导情况及对耳蜗功能的影响。方法制备猪小柱状脱细胞松质骨,与BMP-2复合。选择20只豚鼠,耳后进路进入听泡,左耳植入复合BMP-2的脱细胞松质骨,右耳植入单纯脱细胞松质骨作为对照。分别在植入前、植入即刻、植入后3个月对动物进行ABR测试。并在植入3个月后行组织学及耳蜗基底膜铺片硝酸银染色观察,了解BMP-2诱导成骨情况及对听泡、耳蜗大体结构和毛细胞形态的影响。结果术后动物健康状况良好。植入后即刻,实验耳及对照耳ABR反应阈略有下降,3个月时恢复正常。植入3个月后,耳蜗及听泡大体形态正常,组织学检查可见含BMP-2脱细胞松质骨孔隙内有新骨形成,未见耳蜗及听泡骨质异常增生,镫骨底板关节无固定;硝酸银染色示毛细胞形态正常,无毛细胞缺失。结论缓释BMP-2听泡内植入可诱导新生骨形成,但对动物听泡大体形态无影响,不会引起局部骨质异常增生,对动物听功能无影响,有可能应用于听骨链缺损修复重建。Objective The controlled release BMP-2 and implantation into the acoustic bulla of the guinea-pig and the effects on the cochlea were observed.Methods The acellular cancellous bone was prepared ,accompanied with BMP-2. The material accompanied with BMP-2 was implanted into one acoustic bulla of the animal,and the opposite side of the acoustic bulla was implanted with acellular cancellous bone without BMP-2. A total of 20 guinea-pigs were undergone this procedure. The ABR was tested on the animals before and after the operation immediately and 3 month after operation,respectively. 3 month after operation,the animals were sacrificed. The osteogenesis induced by BMP-2,the acoustic bulla and the cochlea affected by BMP-2 were observed. The structures of hair cells were observed after being treated with silver nitrate. Results The animals recovered soon after surgery. The hearing thresholds of the animals reduced slightly after the operation,and recovered completely 3 month after. The bulla and the cochlea were normal in shape. The osteogenesis occurred in the pore of the acellular cancellous bone with BMP-2. No abnormal hyperplasia of bone in the bulla and the cochlea was found. The articulation between the stapes and oval window was not merged. The shape of the hair cells was normal and no obvious deletion of the hair cells was noted when compared with control group. Conclusion The controlled release BMP-2 could induce osteogenesis in the bulla of the animals. This material did not affect the shape of the bulla and the hearing thresholds. They did not induce any abnormal hyperplasia of bone in the bulla and might be used to reconstruct the affected ossicles.

关 键 词：听骨赝复物 骨形态发生蛋白 耳蜗 反应阈 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]