胃癌组织三叶因子家族的表达与细胞增殖和凋亡的相关性  

Relationship between Expression of Trefoil Factor Family and Gastric Epithelial Cell Proliferation and Apoptosis in Gastric Cancer

作　　者：冯丽英[1] 秦玉彩[1] 马丽[1] 尹希[1] 孙泽明[1] 田惠[1] 

机构地区：[1]河北医科大学第二医院消化内科,石家庄050000

出　　处：《肿瘤防治研究》2010年第1期63-65,共3页Cancer Research on Prevention and Treatment

基　　金：河北省科技攻关资助项目(05276101D-49)

摘　　要：目的探讨三叶因子1(TFF1)、三叶因子2(TFF2)、三叶因子3(TFF3)在胃癌组织中的表达及其与细胞增殖、凋亡的相关性。方法检测40例胃癌组织、20例正常胃黏膜、30例肠上皮化生及24例不典型增生胃黏膜中TFF1、TFF2、TFF3和增殖细胞核抗原(PCNA)的表达及其细胞凋亡情况。结果TFF1、TFF2在正常胃黏膜、不典型增生胃黏膜、肠上皮化生胃黏膜及胃癌组织中均有表达,两者表达强度依次均呈递减趋势(均P<0.05)。TFF3在正常胃黏膜没有表达,而在肠上皮化生胃黏膜、不典型增生胃黏膜和胃癌组织中均有表达,表达强度依次呈递增趋势(P<0.05)。胃癌组织中TFF1、TFF2表达与细胞增殖指数呈负相关(r=-0.55,r=-0.38,均P<0.05),与细胞凋亡指数呈正相关(r=0.58,r=0.48,均P<0.05);TFF3的表达与细胞增殖指数呈正相关(r=0.55,P<0.05),与细胞凋亡指数呈负相关(r=-0.48,P<0.05)。结论TFF可能通过影响胃癌细胞的增殖和凋亡,在胃癌发生、发展中起不同的作用。Objective To investigate the expression of TFF1, TFF2,.TFF3 and to investigate the relationship between TFF1, TFF2, TFF3 and cell proliferation, cell apoptosis in gastric cancen Methods The expression of TFF1 ,TFF2, TFF3 and PCNA were measured by immunohistochemical staining （SP） in gastric cancer（40 specimens）and similar normal gastric mucosa（20 specimens）, intestinal mataplasia in gastric mucosa（30 specimens）, displasic gastric mucosa（24 specimens）, and apoptosis determined by terminal deoxy-nucleotidyl transferase mediated dUTP-biotin nick end labeling（TUNEL）. Results In similar normal gastric rnucosa, intestinal mataplasia mucosa, displasic mucosa and gastric cancer, the levels of TFFI and TFF2 expression and apoptotic index had a decreasing tendency（P〈0. 05, respectively）. By contrast, TFF3 was not detected in similar normal gastric mucosa. The expression level of TFF3 had a increasing tendency in the gastric mucosa of intestinal mataplasia, displasic gastric mucosa and gastric cancer（P〈0. 05）. The level of apoptotic index had an increasing tendency from normal gastric mucosa to intestinal mataplasia mucosa, but decreasing tendency from displasic gastric mucosa to gastric cancer. There was a negative correlation between the expression of TFF1 and TFF2 and proliferating index, but a positive corrolation between the expression of TFF1 and TFF2 and apoptotic index in gastric cancer（both P〈0. 05）. There was a positive correlation between the expression of TFF3 and proliferating index, and a negative correlation between the TFF3 and apoptotic index in gastric cancer. Conclusion TFF might play various roles in the course of gastric cancer via influencing cell proliferation and apoptosis.

关 键 词：胃癌 三叶因子家族 细胞增殖 细胞凋亡 

分 类 号：R392.11[医药卫生—免疫学] R735.2[医药卫生—基础医学]