HLA-DR15与T细胞亚群、免疫球蛋白在再生障碍性贫血和骨髓增生异常综合征中的变化及意义  

Changes of HLA-DR15 and Immunoglobulin,T Lymphocyte Subsets in Patients with Aplastic Anemia,Myelodysplastic Syndrome and Thier Significance

作　　者：崔江霞[1] 裴敏飞[1] 张广森[1] 徐敏[1] 

机构地区：[1]中南大学湘雅二医院血液内科,湖南长沙410011

出　　处：《中国实验血液学杂志》2010年第1期111-115,共5页Journal of Experimental Hematology

摘　　要：本研究旨在探讨再生障碍性贫血(AA)、骨髓增生异常综合征(MDS)患者HLA-DR15表达频率及与疾病的相关性,并分析HLA-DR15与免疫球蛋白、T细胞亚群的关系。采用聚合酶链式反应-序列特异性引物(PCR-SSP)技术检测HLA-DR15的表达,免疫散色比浊法测定免疫球蛋白,流式细胞术检测T细胞亚群。结果显示:AA组、MDS组和正常对照组HLA-DR15表达频率分别为78.6%、63.2%和24.6%,AA组、MDS组与正常对照组比较表达水平有显著性差别(p<0.01),AA组和MDS组的OR值分别为11.262、4.710;正常对照组和血液恶性肿瘤组HLA-DR15表达频率比较无统计学显著差异;AA组、MDS组免疫球蛋白水平和T细胞亚群比例异常在HLA-DR15阳性组与阴性组之间比较无显著性差异(p>0.05)。结论:AA、MDS患者HLA-DR15表达频率显著高于正常人及血液恶性肿瘤患者,AA、MDS组OR值均>1,说明疾病与HLA-DR15呈正相关,HLA-DR15可能是AA、MDS的易感基因;AA和MDS常见免疫球蛋白水平和T细胞亚群比例异常,但与HLA-DR15的表达无明显关系。The objective of this study was to detect the expression frequency of HLA-DR15 in patients with aplastic anemia（AA） and myelodysplastic syndrome（ MDS ）, to investigate the relation of expression frequency with diseases and to analyze the relationship between immunoglobulin, T lymphocyte subsets and HLA-DR15. HLA-DR15 expression was detected by PCR-SSP; immunoglobulin was detected by immune turbidimetry; T cell subsets were detected by flow cytometry. The results showed that the expression rates of HLA-DR15 in AA and MDS as well as normal control groups were 78.6% ,63.2% and 24.6% respectively. The difference between AA, MDS and the normal control groups was statistically significant （p 〈 0.01 ）. OR （ odds ratios ） values of AA and MDS groups were 11, 262,4.710 respectively. Compared with normal control group, expression rate of HLA-DR15 in hematologic malignancy group was not significantly different. The immunoglobulin level and abnormal T cell subsets in AA and MDS groups were statistically different between HLA-DR15 positive and negative groups （p 〉 0.05 ）. It is concluded that the frequency of HLA-DR15 antigen in AA and MDS patients is significantly higher than that in normal control and hematologic malignancy group. OR value 〉 1 showed a positive correlation between the diseases and HLA-DRIS. HLA-DR15 is a susceptible gene in AA and MDS. The abnormalities of immunoglobulin level and ratios of T cell subsets in AA and MDS are common, but are not associated significantly with the expression of HLA-DR15.

关 键 词：再生障碍性贫血 骨髓增生异常综合征 人类白细胞抗原 免疫球蛋白 T细胞亚群 

分 类 号：R556.5[医药卫生—血液循环系统疾病] R733.3[医药卫生—内科学]