慢性特发性血小板减少性紫癜患者活化T细胞CD30表达与凋亡关系  

Relationship between Apoptosis and CD30 Expression of Activated T-lymphocyte in Patients with Chronic Idiopathic Thrombocytopenic Purpura

作　　者：张耀[1] 陈琦[1] 方宁[2] 

机构地区：[1]遵义医学院附属医院血液科,贵州遵义563003 [2]遵义医学院细胞工程实验室,贵州遵义563003

出　　处：《中国实验血液学杂志》2010年第1期195-198,共4页Journal of Experimental Hematology

摘　　要：本研究检测慢性特发性血小板减少性紫癜(ITP)患者外周血单个核细胞中CD3+数、CD3+/CD30+T细胞比值和活化后T细胞凋亡率及外周血血小板计数,探讨2组间的差异和患者活化T淋巴细胞CD30表达率与凋亡率和血小板计数的相关性。以40例慢性ITP患者为研究对象,24例健康志愿者为正常对照,体外培养刺激T细胞活化后运用流式细胞仪检测外周血单个核细胞悬液中CD3+数、CD3+/CD30+T细胞比值及CD3+T细胞凋亡率,同步检测血小板计数,分析ITP组与对照组各指标间的差异和相关性。结果表明:ITP组外周血CD3+细胞数与对照组无明显差异(p>0.05)。ITP组活化T细胞表面CD30表达率明显高于对照组(p<0.01)。ITP组活化T细胞凋亡率明显低于对照组(p<0.01)。活化后T细胞表面CD30表达与其凋亡率呈负相关(r=-0.786,p<0.01)。活化T细胞凋亡率与血小板计数呈正相关(r=0.680,p<0.01)。结论:慢性ITP患者活化T细胞CD30的高表达导致了T细胞活化后凋亡受阻,在体内蓄积引起免疫功能紊乱,使血小板减少。This study was aimed to detect the CD3＋ count, ratio of CD3＋ / CD30＋ T-lymphocytes, the apoptosis rate of activated T-lymphocytes in peripheral blood mononuclear ceils and the circulating platelet counts in patients with chronic idiopathic thrombocytopenic purpura （ITP）, and to investigate the relationship between them. 40 cases of chronic ITP patients were taken as the research group and 24 healthy persons were served as the control group. The CD3 count, ratio of CD3/CD30 T-lymphocytes and the apoptosis rate of activated T-lymphocytes were detected by flow cytometry, the platelet count was measured at the same time. The difference between 2 groups and correlation of the activated T lymphocyte CD30 expression rate with apoptotic rate and platelet count in patients were analysed. The results showed that there was no significant difference in the count of peripheral blood CD3＋ cell between the research group and the control group （p 〉0.05）. The expression of CD30 on activated T-lymphocytes in ITP group was obviously higher than that in control group （p 〈 0.01 ）. The apoptosis rate of activated T-lymphocyte in ITP group was remarkably lower than that in control group （p 〈 0.01 ）. The expression of CD30 on activated T-lymphocyte exhibited a negative correlation with the apoptosis rate （ r = - 0. 786, p 〈 0.01 ）. The apoptosis rate of activated T-lymphocyte was positively correlated with the platelet count of peripheral blood （ r = 0. 680, p 〈 0.01 ）. It is concluded that the high expression of CD30 on activated T-lymphocytes in patients with chronic ITP results in the inhibition of apoptosis, which leads to immune disorder and thrombocytopenia.

关 键 词：特发性血小板减少性紫癜 T淋巴细胞 CD30 细胞凋亡 

分 类 号：R554.6[医药卫生—血液循环系统疾病] R733.71[医药卫生—内科学]