中药黄芪可改善肥胖大鼠内皮依赖性血管舒张功能  

Astragalus membranaceus improves endothelial-dependent vasodilator function in obese rats

作　　者：李明锐[1] 余叶蓉[1] 邓刚[1] 

机构地区：[1]四川大学华西医院内分泌科,四川成都610041

出　　处：《南方医科大学学报》2010年第1期7-10,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30570874)

摘　　要：目的研究黄芪对高脂饲料喂养的肥胖大鼠离体胸主动脉内皮依赖性血管舒张功能的影响。方法(1)在体研究,SD大鼠随机分为正常对照组、肥胖组、黄芪干预组(高脂饲料喂养同时每天灌胃黄芪液),6周后处死所有大鼠,留取并称量所有大鼠的腹腔内脂肪。采用离体血管环灌流方法,观察各组大鼠离体主动脉环对乙酰胆碱或硝普钠的舒张反应;(2)离体研究,正常对照组和肥胖组各5只,以黄芪液孵育离体血管环,并与空白对照比较,观察黄芪对离体血管环舒张功能的影响。结果肥胖组体脂比较正常组明显增加,黄芪液灌胃6周明显减少肥胖组动物腹部脂肪堆积。肥胖组内皮依赖性血管舒张功能较正常对照组明显下降,黄芪液灌胃6周可部分改善肥胖组动物内皮依赖性血管舒张功能,各组非内皮依赖性血管舒张功能无明显差别。肥胖组离体血管环经黄芪液孵育3h后,其受损的内皮依赖性血管舒张功能可明显改善。结论黄芪可改善高脂饲养喂养的肥胖大鼠受损的内皮依赖性血管舒张功能,其机制可能与缓解胰岛素抵抗以及直接促使内皮细胞NO产生增多有关。Objective To investigate the effect of Astragalus membranaceus （AM） on endothelial-dependent （EDV） and nondependent （EIV） vascular relaxation in ex vivo thoracic aortic rings of obese rats. Methods Fifteen SD rats were randomized into 3 equal groups, namely the control group fed with normal chow, obese group with high-fat chow, and AM intervention group fed with high-fat chow and daily AM gavage. The rats were sacrificed after 6 weeks of feeding, and the aortic rings were dissected and cut into 3-mm rings. The response to acethylcholine （Ach） and sodium nitroprusside （SNP） were examined in organ bath. In ex vivo study, the aortic rings obtained from the control group and obese group were incubated with AM or vehicle for 3 h in organ bath before testing the EDV and EIV. The body weight and weight of the visceral fat in each group were recorded. Results The weight of visceral fat was greater in the obese group than in the control group, and a 6-week AM treatment significantly reduced the fat tissue due to high-fat diet. The maximum EDV value was （87.0±3.5）% in the control group, （54.8±7.8）% in the obese group, and （69.8±5.7）% in AM intervention group; the EIV values were comparable between the 3 groups. After incubation with AM, the maximum EDV values of aortic rings obtained from the obese group were significantly increased from （55.6%±8.3）% to （85.1±4.5）%. Conclusion AM can improve endothelial dysfunction in obese rats, and the mechanism involves improved insulin resistance and increased endothelium-derived NO productor function.

关 键 词：黄芪 肥胖 胰岛素抵抗 内皮依赖性血管舒张功能 

分 类 号：R285.5[医药卫生—中药学]