腺病毒介导的人基质细胞衍生因子-1对心肌梗死大鼠心室重构的影响  

Effect of adenovirus-mediated stromal cell-derived factor-α gene transfer on ventricular remodeling in rats with myocardial infarction

作　　者：杨建业[1] 张迎春[1] 唐俊明[1,2] 安庆宝[2] 郭凌郧[1] 孔霞[1] 黄永章[1] 郑飞[1] 王家宁[1] 

机构地区：[1]郧阳医学院附属人民医院临床医学研究所,湖北十堰442000 [2]郧阳医学院实验动物中心、生理学教研室,湖北十堰442000

出　　处：《南方医科大学学报》2010年第1期38-42,46,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(30700306);湖北省卫生厅(JX3B29);湖北省自然基金(2005ABA079)

摘　　要：目的探索腺病毒介导的人基质细胞衍生因子-1对心肌梗死心室重构的影响。方法利用细菌内同源重组快速构建重组腺病毒质粒和制备hSDF-1α重组腺病毒(AdV-EGFP-hSDF-1α,Ad-SDF-1)。采用成年SD大鼠,经左冠状动脉前降支结扎建立急性心肌梗死模型。术后1h,将1×1010PFU AdV-SDF-1分6个点注射到心肌梗死部位及其周边区域。移植后4周测定心脏功能,随后取材、连续冰冻切片,观察心肌组织形态学、心肌梗死部位胶原含量的变化以及心室壁厚度的变化。结果AdV-SDF-1组心室壁明显增厚,胶原含量明显减少,心室膨胀程度明显降低,心脏功能改善。结论SDF-1过表达通过抑制心肌梗死部位胶原的合成与沉积,延缓心室重构,达到改善心脏功能的目的。Objective To explore the effect of adenovirus-mediated human stromal cell-derived factor-1α （hSDF-1α） on ventricular remodeling in rats with myocardial infarction. Methods A recombinant adenoviral plasmid containing hSDF-1α cDNA was constructed using homologous recombination in bacteria and the recombinant adenovirus particles expressing hSDF-1α （AdV-SDF-1） were prepared. In rat models of myocardial infarction induced by left anterior descending artery occlusion, 1×1010 PFU AdV-SDF-1 or PFU AdV-LacZ were injected at multiple sites into the infarcted myocardium 1 h after the operation, using 200 l cell-free PBS as the control. Four weeks after the injection, the cardiac function of the rats was analyzed, and the heart tissues were taken after the measurement of hemodynamics. On serial frozen sections, histological observation and morphometric measurement were carried out using a microscopic image analysis system, and the expression of hSDF-1α was detected by immunocytochemistry. Results Four weeks after AdV-SDF-1 injection, the myocardium in the infracted area showed significantly higher expression rates of hSDF-1α. The injection resulted in a obvious reduction in the infarct size and collagen content and a marked increase in the left ventricle wall, and the rats showed improved cardiac functions. Conclusion SDF-1α can improve the cardiac structure and function in rats with myocardial infarction by inhibiting collagen synthesis and deposition in the infarcted area.

关 键 词：人基质细胞源衍生因子-1 腺病毒 心肌梗死 心室重构 

分 类 号：R542.22[医药卫生—心血管疾病]