甘氨酸对新生大鼠离体延髓脑片吸气神经元放电活动的影响  

Effects of glycine on the discharge activities of the inspiratory neurons in neonatal rat medulla oblongata slices

作　　者：程静[1] 吴敏[2] 吴中海[1] 

机构地区：[1]南方医科大学生理学教研室 [2]南方医院中医科,广东广州510515

出　　处：《南方医科大学学报》2010年第1期51-53,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30570670);广东省自然科学基金(5004714)

摘　　要：目的探讨甘氨酸(Gly)对延髓脑片面神经后核内侧区(mNRF)吸气神经元放电活动的影响。方法制作新生大鼠离体延髓脑片标本,主要包含mNRF,并完整保留舌下神经根,以改良Kreb,s液灌流脑片,同步记录舌下神经根呼吸节律性放电活动(RRDA)和吸气神经元的放电活动。在灌流液中分别单独给予Gly受体的特异性激动剂Gly和特异性拮抗剂士的宁(STR),并分别观察其对神经元放电活动的影响。结果给予Gly受体激动剂Gly后,吸气神经元的吸气时程(TI)缩短,放电的积分幅度(IA)减小,单位放电频率(PFn)变慢。给予其拮抗剂STR后,吸气神经元的呼气时程(TE)和呼吸周期(RC)缩短,PFn无明显改变。结论Gly及其受体参与了呼吸节律的调节,其调节作用可能是通过影响吸气神经元的放电活动参与了呼吸时相的转换。Objective To observe the effect of glycine on the discharge activities of inspiratory neurons in the medulla oblongata slices of neonatal rats. Methods Neonatal rat medulla oblongata slices including the medial region of the nucleus retrofacialis （mNRF） with the hypoglossal nerve rootlets retained were prepared. The activity of the inspiratory neurons in the mNRF and the respiratory rhythmical discharge activity （RRDA） of the hypoglossal nerve rootlets were simultaneously recorded using microelectrodes and suction electrodes, respectively. The effect of glycine on the rhythmical respiration was investigated by administration of glycine receptor agonist and its specific antagonist strychnine （STR） into a modified Kreb＇s perfusion solution （MKS）. Results Glycine significantly shortened the inspiratory time （TI） and integral amplitude （IA） of the inspiratory neurons and decreased the peak frequency （PFn）. By contrast, STR induced a decrease in the expiratory time （TE） and respiratory cycle （RC） of the inspiratory neurons, but the peak frequency was not significantly affected. Conclusion Glycine and its receptor play a role in the modulation of the rhythmical respiration, and glycine is probably involved in the phase-switching between expiration and inspiration by affecting the inspiratory neurons.

关 键 词：甘氨酸 面神经后核内侧区 呼吸节律 吸气神经元 

分 类 号：R338.2[医药卫生—人体生理学]