SARS-CoV功能性受体血管紧张素转换酶2在灵长类动物肾脏上皮细胞的表达(英文)  

Expression of SARS-CoV functional receptor angiotensin-converting enzyme 2 in nephritic epithelium of primates

作　　者：杨秀红[1,2] 秦川[1] 陈云新[1] 朱华[1] 黄澜[1] 刘亚莉[1] 马春梅[1] 张连元[2] 杨方[2] 

机构地区：[1]中国协和医科大学实验动物研究所,北京100021 [2]华北煤炭医学院生理学教研室,唐山063000

出　　处：《中国病理生理杂志》2010年第1期158-162,共5页Chinese Journal of Pathophysiology

基　　金：Supported by Basic Scientific Research Operation Cost of State-leveled Public Welfare Scientific Research Courtyard(No. DWS200728)

摘　　要：目的:研究血管紧张素转换酶2(ACE2)在灵长类动物肾脏上皮细胞上的表达。方法:体外培养非洲绿猴肾细胞VeroE6,利用RT-PCR及免疫细胞化学的方法检测细胞ACE2的表达;利用免疫组织化学方法检测2例恒河猴和2例正常人肾组织ACE2的表达分布。结果:RT-PCR显示,从VeroE6细胞提取的总RNA经逆转录可检测出ACE2的扩增条带;免疫细胞化学方法检测到VeroE6细胞胞浆内反应明显阳性;ACE2主要分布在恒河猴和人肾组织近端肾小管上皮细胞的管腔膜和胞浆。结论:ACE2在灵长类动物肾脏上皮细胞的核酸水平和蛋白水平均有表达,主要位于细胞的管腔膜和胞浆;肾脏上皮细胞表达的ACE2为SARS-CoV的入侵提供了条件,有可能是SARS患者并发肾功能损伤的原因之一。AIM ： To investigate the expression of angiotensin - converting enzyme 2 （ACE2） in nephritic epithelium of primates. METHODS： The expression of ACE？. in Vero E6 cells was detected by the techniques of RT - PCR and immunocytochemistry （ICC） techniques. The distribution of ACE2 protein in kidney tissues of two Rhesus monkeys and two normal human cases was observed by immunohistochemistry （IHC） techniques. RESULTS： Vero E6 cells were found to express both ACE2 mRNA and protein. ACE2 protein was mainly located in epithelium of proximal tubules of Rhesus monkey and human kidney. CONCLUSION： The expression of ACE2 in epithelium of primate kidney may provide the condition for SARS - CoV entry, which may be one of the reasons for inducing renal damage in SARS patients.

关 键 词：血管紧张素转换酶2 SARS—CoV 灵长类 肾 

分 类 号：R563[医药卫生—呼吸系统]