耐甲氧西林金黄色葡萄球菌检测方法的评估  

Evaluation of four techniques for detection of meticillin-resistant Staphylococcus aureus

作　　者：王爱玲[1] 付明霞[2] 宋向芹[1] 安新业[1] 赵红梅[1] 

机构地区：[1]滨州医学院附属医院检验科,滨州市256603 [2]滨州市人民医院检验科

出　　处：《滨州医学院学报》2009年第6期408-409,412,共3页Journal of Binzhou Medical University

基　　金：山东省自然科学基金(ZR2009CL002)

摘　　要：目的评价4种检测耐甲氧西林金黄色葡萄球菌(MRSA)方法的临床应用价值。方法对94株临床分离的金黄色葡萄球菌采用苯唑西林纸片扩散法、头孢西丁纸片扩散法和苯唑西林微量稀释法筛选MRSA,并采用聚合酶链反应(PCR)检测m ecA基因作为检测MRSA的金标准。结果94株中有63株为MRSA,检出率为67%;其中头孢西丁纸片扩散法筛选MR-SA敏感性为100.0%,特异性100.0%;苯唑西林K-B法筛选MRSA敏感性为96.8%,特异性为87.1%;苯唑西林微量稀释法筛选MRSA敏感性为98.4%,特异性为96.8%。结论综合分析头孢西丁纸片扩散法可作为医院微生物实验室日常工作中检测MRSA的简便又准确的实验方法。Objective To evaluate the clinical application of the four methods for detection of meticillin-resistant Staphylococcus aureus.Methods Disk diffusion method with cefoxitin and oxacillin,the microdilution method of oxacillin and PCR amplification of the mecA gene as the reference method were performed to analyze 94 clinical isolates of Staphylococcus aureus.Results 63 strains were MRSA,the cefoxitin disk test（specificity100.0%,sensitivity 100.0%）was superior to the oxacillin disk method（specificity 87.1%,sensitivity 96.8%） and the microdilution method of oxacillin（specificity 96.8%,sensitivity 98.4%）.Conlusion The cefoxitin disk test is simple and reliable for routine detection and confirmation of MRSA in the clinical laboratories.

关 键 词：金黄色葡萄球菌 头孢西丁 苯唑西林 MECA基因 

分 类 号：R378.1[医药卫生—病原生物学]