胎鼠前角运动神经元的取材与培养研究  

The culture of anterior horn motor neurons of embryo rats

作　　者：孙毅[1] 赵冬梅[1] 张烨[1] 刘洪付[1] 黄飞[1] 

机构地区：[1]滨州医学院人体解剖学教研室,烟台市264003

出　　处：《滨州医学院学报》2009年第6期420-421,424,共3页Journal of Binzhou Medical University

基　　金：滨州医学院科技计划(BY2006KJ09);滨州医学院大学生科技创新课题(BY2007DKCX12)

摘　　要：目的探讨脊髓前角运动神经元的取材时间、方法和体外培养。方法取胎龄为14~18 d的SD大鼠胚胎及新生鼠脊髓腹侧半,分别采用DMEM/F12和Neurobasal-A培养基进行培养,观察前角运动神经元的生长状态。结果胎龄15~16 d的胎鼠易取材,联合Neurobasal-A＋2%B27培养基培养的前角运动神经元细胞状态好、纯度高。结论采用胎龄15~16 d的胎鼠脊髓腹侧半,联合Neurobasal-A＋2%B27培养基培养细胞能获得高纯度的前角运动神经元,为研究运动神经元疾病打下实验基础。Objective To observe the appropriate time and method of obtaining anterior horn motor neurons,and discuss the method of culture in vitro.Methods The anterior horn motor neurons of SD rat were obtained from 14-18 d embryos or from neonatal rats,and they were cultured by DMEM/F12 or Neurobasal-A in vitro.After culture,the grow condition of the anterior horn motor neurons was observed.Results The anterior horn motor neurons were easily obtained from 15-16 d embryos,and the cells cultured by Neurobasal-A ＋ 2% B27 were in the best condition.Conclusion The 15-16 d embryos is the best material to obtain anterior horn motor neurons,and the Neurobasal-A ＋ 2% B27 is the best medium for anterior horn motor neurons.

关 键 词：前角运动神经元 胎鼠 细胞培养 

分 类 号：R322-33[医药卫生—人体解剖和组织胚胎学]