胰岛素对糖尿病大鼠视网膜VEGF和Ang-2表达的影响  

The Effect of Insulin Therapy on Expression of VEGF and Ang-2 in Diabetic Rat Retina

作　　者：马萍萍[1] 王玉粦 吴炎 李斌[3] 林少芬[3] 唐仕波[3] 

机构地区：[1]广东省东莞市人民医院眼科,523018 [2]广东省深圳市人民医院内分泌科,518020 [3]中山大学中山眼科中心,广州510060

出　　处：《医学研究杂志》2010年第1期61-63,共3页Journal of Medical Research

基　　金：深圳市科技计划项目(200404048)

摘　　要：目的观察胰岛素治疗对糖尿病大鼠视网膜血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)和血管生成素-2(angiopoietin-2,Ang-2)表达的影响,探讨胰岛素在糖尿病视网膜病变发生发展中的作用。方法链尿佐菌素(streptozotocin,STZ)诱导糖尿病大鼠动物模型,成模后经胰岛素治疗控制血糖,随机分为糖尿病血糖严格控制组、糖尿病血糖非严格控制组、糖尿病组和正常对照组。应用免疫荧光组织化学染色法检测大鼠视网膜组织VEGF、Ang-2的表达,用计算机-图像分析软件对VEGF、Ang-2免疫荧光强度进行半定量分析。结果与正常对照组比较,糖尿病组大鼠视网膜VEGF、Ang-2平均荧光强度分别增加了2.38倍和2.41倍(P<0.05)。与糖尿病组比较,血糖严格控制组大鼠视网膜VEGF、Ang-2的表达分别下降了1.47倍和1.51倍(P<0.05),而血糖非严格控制组VEGF、Ang-2的表达无明显下降(P>0.05)。结论胰岛素治疗严格控制血糖可降低糖尿病大鼠视网膜VEGF、Ang-2的表达,对糖尿病视网膜病变的发生发展有保护作用。ObjectiveTo observe the changes of VEGF and Ang-2 expression in streptozotocin（STZ）-induced diabetic rat retina after insulin therapy and explore possible roles of insulin in the development of diabetic retinopathy. MethodsDiabetes was induced in 8-week-old male wistar rats by a single intraperitoneal injection of STZ. After three weeks, animals were randomly divided into four groups：（1）diabetic rats received intensive insulin therapy for 20 days;（2）diabetic rats received unregular insulin therapy, which caused the abrupt fluctuation of glycemic level;（3）diabetic control rats; and （4）normal control rats. After treatment, the animals were sacrificed with an overdose of anesthesia,and the eyes were enucleated and fixed in 4% paraformaldehyde immediately. Paraffin sections of retina were prepared.Expression of VEGF and Ang-2 was assessed by immunofluorescence stain and images analysis. ResultsQuantitative analysis showed VEGF and Ang-2 protein expression was increased by 2.38-fold and 2.41-fold in diabetic rats retinas as compared to non-diabetic rats retinas respectively （P〈0.05）. The expression of VEGF and Ang-2 was decreased by 1.47-fold and 1.51-fold in diabetic rats which received intensive therapy respectively （P〈0.05）. And there were no significant decreases in VEGF and Ang-2 expression in diabetic rats which received unregular insulin therapy（P〉0.05）. ConclusionIntensive insulin therapy could decrease VEGF and Ang-2 expression in retina and has protective effect on diabetic retinopathy in STZ-diabetic rats.

关 键 词：糖尿病视网膜病变 胰岛素 血管内皮细胞生长因子 血管生成素-2 

分 类 号：R587.2[医药卫生—内分泌] R587.1[医药卫生—内科学]