四环素眼膏微生物限度检查法研究  

Study on Microbial Limit Test Method for Tetracycline Ointment

作　　者：林丽英[1] 黄灿[1] 

机构地区：[1]广东省药品检验所,广州510180

出　　处：《医学研究杂志》2010年第1期108-110,共3页Journal of Medical Research

基　　金：广东省药品检验所资助项目(SN20076102)

摘　　要：目的建立四环素眼膏的微生物限度检查法。方法采用培养基稀释法、萃取+薄膜过滤法,测定四环素眼膏对5种试验菌株的回收率,并对控制菌检查法进行验证。结果真菌及酵母菌数采用培养基稀释法检查,细菌数、控制菌可采用萃取+薄膜过滤法检查。结论该方法可有效检出四环素眼膏中污染的微生物,为更好地评价四环素眼膏的有效性和质量的控制提供科学的依据。ObjectiveTo construct the microbial limit test method for tetracycline ointment. MethodsThe medium dilution method and extracting binding membrane filtration method were used in the recovery rate and control bacterial test of tetracycline ointment. ResultsThe counting of mould and yeast could be tested by the medium dilution. Extracting binding membrane filtration method could help to bacterial counting and detection of control bacterial. ConclusionThe method can detect the microbial contamination of tetracycline ointment effectively, which supplies the scientific data for the better evaluation of the validity and quality control to tetracycline ointment.

关 键 词：四环素眼膏 微生物限度检查 方法研究 

分 类 号：R978.7[医药卫生—药品] R988.1[医药卫生—药学]