γ射线辐照外周血单个核细胞联合ApoG2体外杀伤PC-3细胞的效应  

Studies on the Killing Effect of γ Ray Irradiated Peripheral Blood Mononuclear Cells Combined with ApoG2 on Human Prostate Cancer PC-3 Cells

作　　者：白艳丽[1] 龚卫锋[1] 王兆龙[1] 李静[1] 李珊[1] 刘志新[2] 张献清[2] 

机构地区：[1]西安市中心医院输血科,陕西西安710003 [2]第四医大学西京医院输血科,陕西西安710032

出　　处：《中国医学物理学杂志》2010年第1期1676-1680,共5页Chinese Journal of Medical Physics

基　　金：陕西省科学技术研究发展计划项目课(NO.2008K09-09)

摘　　要：目的:观察低剂量辐射外周血单个核细胞联合棉酚衍生物ApoG2对体外培养人前列腺癌PC-3细胞的杀伤作用。方法:采用1Gyγ射线照射人外周血单个核细胞,辐射剂量率为17Gy/min,实验设对照组、照射及未照射的外周血单个核细胞与PC-3细胞共培养组、ApoG2处理组及照射的外周血单个核细胞与ApoG2联合作用组。利用吖啶橙/溴化乙啶(AO/EB)荧光双染色法及MTT法,观察外周血单个核细胞和/或ApoG2对肿瘤细胞的杀伤情况。结果:辐照的外周血单个核细胞及ApoG2处理组对PC-3的杀伤作用明显增强,杀伤活性明显高于未辐照组(P<0.05),而联合作用组的杀伤活性明显高于辐照组及ApoG2处理组((P<0.01)。结论:低剂量辐射外周血单个核细胞联合ApoG2可以明显提高其体外的抗肿瘤效应。Objective： To observe the killing effect of irradiated peripheral blood mononuclear cells （PBMCs） at low dose combined with apogossypolone （ApoG2） on cultured human prostate cancer PC-3 cells. Methods： Human PBMCs were irradated by gamma my at 1 gray,the irradiation dose rate was 17 Gy/min.The experiment were divided into PC-3 tumor cell control group,PC-3 cells with irradiated and non-irradiated PBMCs co-culture groups, ApoG2 treatment group, irradiated PBMCs and ApoG2 co-treatment group.Acridine orange/ethidium bromide （AO/EB） staining and MTT method were used to observe the killing effect of PBMCs and/or ApoG2. Results： The killing activity of irradiated PBMCs group and ApoG2 treatment group were obviously increased and were higher than that of non-irradiated group （P〈O.05）. The killing activityof combined group were much higher than that of irradiated group and ApoG2 treatment group （P 〈0.01）. Conclusion： Irradiated PBMCs at low dose combined with ApoG2 can enhances the anti-tumor effects markedly.

关 键 词：外周血单个核细胞 酌射线 低剂量辐射 前列腺癌 ApoG2 

分 类 号：R311.1[医药卫生—基础医学] R811.5