毛细管电泳法间接测定茶水、矿泉水中金属离子  

Separation and Determination of Some Metal Ions in Tea and Mineral Water Using Capillary Electrophoresis

作　　者：王清萍[1,2] 陈国南[2] 林金明[3] 

机构地区：[1]福州大学化学化工学院食品安全分析与检测教育部重点实验室,福建福州350002 [2]福建师范大学化学与材料学院,福建福州350007 [3]清华大学化学系,北京100084

出　　处：《分析测试技术与仪器》2009年第3期135-139,共5页Analysis and Testing Technology and Instruments

基　　金：福建省自然科学基金资助项目(2007J0130);福州大学人才基金

摘　　要：采用毛细管电泳间接紫外检测技术测定了茶水、矿泉水中金属离子的含量.研究了电泳液组成和最佳实验条件.结果表明：采用pH=4.82含有浓度为6.0 mmol/L咪唑的乙酸缓冲液作为电泳液,压力进样（30 mbar×5 s）,未涂层石英毛细管柱75μm×50 cm,分离电压12 kV,检测波长210 nm,柱温为25℃实验条件下,6 min内实现K＋、Ca2＋、Na＋、Mg2＋、Zn2＋5种离子的分离和测定.保留时间的相对标准偏差（RSD）小于0.5%,峰面积RSD小于5%（n=7）,最低检测限依次为0.002、0.001、0.001、0.010和0.050 mg/L.该方法用于茶水、矿泉水中金属离子含量的测定,回收率95.6%-108.6%,结果良好.Capillary zone electrophoresis（CZE） was used to determine metal ions in Longjing Tea and Baiyan mineral water.6 mmol/L imidazol solution was used as the background electrolyte and 0.2 mol/L acetic acid was used as the selectivity improvement reagent.Under conditions of pH 4.82,12 kV separation voltage,25 ℃ column temperature and indirect UV（210 nm） detection,K＋,Ca2＋,Na＋,Mg2＋ and Zn2＋ were separated and determined within only 6 min.The relative standard deviations for retention times and peak areas were less than 0.5% and 5%（n=7）,respectively.The detection limits of K＋,Ca2＋,Na＋,Mg2＋ and Zn2＋ were 0.002,0.001,0.001,0.010 and 0.050 mg/L respectively.The recoveries of the metal ions in real samples were in the range of 95.6%-108.6%.

关 键 词：毛细管电泳 间接检测 金属离子 

分 类 号：O657.7[理学—分析化学]