一氧化氮与门脉高压性肠病关系的实验研究  

Experimental study on the relationship of nitric oxide with portal hyperten- sive enteropathy

作　　者：余云波[1] 尹朝礼[1] 余金生[1] 梁扩寰[1] 

机构地区：[1]同济医科大学同济医院

出　　处：《中华消化杂志》1998年第4期197-200,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金

摘　　要：目的探讨ＮＯ在门脉高压性肠病发病机理中的作用。方法制作大鼠四氯化碳门脉高压模型。第１２周末，在冰冻切片上进行结肠ＮＡＤＰＨ黄递酶组织化学染色；应用化学发光法测定ＮＯＳ活性；ＲＴ－ＰＣＲ反应测定ＮＯＳｍＲＮＡ表达量。结果（１）ＮＡＤＰＨ黄递酶组化染色：模型组大鼠结肠粘膜下小血管内皮细胞和神经纤维的ＮＯＳ染色强度显著高于正常对照组。而表层上皮细胞ＮＯＳ染色减弱。（２）化学发光法测定ＮＯＳ活性：模型组大鼠结肠粘膜ＮＯＳ活性较正常对照组显著增加，其中ｉＮＯＳ活性增加更为明显。（３）ＲＴ－ＰＣＲ：模型组大鼠结肠粘膜ＮＯ－ＳｍＲＮＡ表达量较正常对照组显著增加。结论ＮＯ可能由于其扩张血管的特性，而参与门脉高压性肠病血管病变的发病过程，并与门脉高压性肠病粘膜病变、可能存在的运动功能异常亦有一定的关系。Objective To probe the role of nitric oxide(NO) in the pathogen- sis of portal hypertensive enteropathy (PHE). Methods Cirrhotic portal hyperten- sion was induced in rats by percutaneous administration of carbon tetrachloride. At the end of 12th week, NADPH-diaphorase staining was performed on ice frozen section with the sample of fresh colonic tissues; NO synthase (NOS) activities and N0S mRNAs of coloniic mucosal tissues were investigated with chemo-luminescence method and reverse transcription-polymerase chain reaction (RT-PCR) , respective- ly. Results (1)NADPH-diaphorsae histochemical staining: With the computer image analysis and paired test, NOS staining intensities of submucosal vascular endothelial cell (P<0.01) and nerve fiber (P<0.05) were all significantly higher in PHE group than those of normal, but that of superficial epithelial cells was significantly lower (P<0.01). (2)Chemoluminescence method: General NOS activity of colonic mucosa was significantly higher in PHE group than that in normal group (P<0.01). Moreover, the degree of increase of iNOS activity was greater than one of cNOS (104 % vs 35 % ). (3)RT-PCR: NOS mRNA expression was remarkably higher in PHE group than that in control group (P<0.01). Conclusion The above results show vasodilation by NO may be involved in the pathogenesis of vascular lesions of PHE in cirrhotic rats, and may also correlate in some degree with mucosal lesions and abnormal colonic motor functions in PHE .[

关 键 词：一氧化氮 门静脉高压 肠疾病 病理学 

分 类 号：R657.340.6[医药卫生—外科学] R574.02[医药卫生—临床医学]