胡颓子叶正丁醇部位化学成分及其细胞毒活性初步研究  

Primary Studies on Chemical Constituents from Butanol Extraction of the Thorny Elaeagnus Leaf and Their Cytotoxicity

作　　者：付义成[1] 王晓静[1] 贾献慧[1] 周玲[1] 

机构地区：[1]山东省医学科学院药物研究所/山东省现代医用药物与技术重点实验室,山东济南250062

出　　处：《中药材》2009年第12期1848-1850,共3页Journal of Chinese Medicinal Materials

基　　金：山东省自然科学基金资助项目(Y2008C171)

摘　　要：目的:研究胡颓子叶正丁醇萃取部位的化学成分及其对人宫颈癌、人胃癌细胞的细胞毒活性。方法:采用反复柱色谱方法进行分离,利用化合物的光谱学数据结合理化性质鉴定化合物的结构;采用MTT法测定化合物对体外肿瘤传代细胞SGC-7901(人胃癌)和Hela(人宫颈癌)的细胞毒活性。结果:分离并鉴定了4个化合物:3′-甲氧基槲皮素(Ⅰ)、山柰酚-3-O-β-D-6-O-(对羟基桂皮酰基)-吡喃葡萄糖苷(Ⅱ)、葛花苷(Ⅲ)、3′-O-甲基鞣花酸-4-O-α-L-吡喃鼠李糖苷(Ⅳ)。4个化合物对体外肿瘤传代细胞SGC-7901(人胃癌)和Hela(人宫颈癌)有一定的抑制作用。结论:化合物Ⅰ、Ⅲ、Ⅳ为首次从该种植物中分离得到。化合物Ⅰ和Ⅱ对Hela细胞株有明显的细胞毒作用。Objective：To study the chemical constituents and test the cytotoxicity of the purified compounds from the Thorny Elaeagnus Leaf.Methods：The constituents were isolated by column chromatography and their structures were elucidated by chemical properties and spectroscopic analysis.The cytotoxicity of the chemical components for SGC-7901 and Hela cells was measured by MTT method.Results：Four components were isolated and identified as 3＇-O-methylquercetin（1）,kaempferol-3-O-β-D-6-O-（p-hydroxycinnamoyl） -glucopyranoside（2）,kakkalide（3）,3＇-O-methyl-ellagic acid-4-O-α-L-rhamnicoside（4）.These components had cytotoxicity for SGC-7901 and Hela cells.Conclusion：Compounds 1,3 and 4 are isolated from Thorny Elaeagnus Leaf for the first time.The results of pharmacological experiments indicate that compounds 1 and 2 show strong cytotoxicity to Hela cells.

关 键 词：胡颓子叶 化学成分 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]