雷丸菌丝蛋白对H_(22)荷瘤小鼠的肿瘤抑制及免疫调节作用  

Effect of Proteins Extracted from Mycelia of Omphalia lapidescens on Inhibiting H_(22) Liver Cancer in Mice and Regulating Immune Function

作　　者：陈宜涛[1] 林美爱[2] 程东庆[1] 石志坚[1] 朱健灵[1] 吴剑[1] 

机构地区：[1]浙江中医药大学生命科学学院,浙江杭州310053 [2]浙江中医药大学研究生处,浙江杭州310053

出　　处：《中药材》2009年第12期1870-1874,共5页Journal of Chinese Medicinal Materials

基　　金：浙江省自然科学基金项目(Y207765);浙江省教育厅科研项目(20050817);浙江省中医药科技计划项目(2007YA005)

摘　　要：目的:提取雷丸菌丝蛋白,检测其对H_(22)肝癌细胞荷瘤小鼠的肿瘤抑制作用。方法:随机将50只ICR小鼠分为5组,分别为模型组、环磷酰胺组及雷丸菌丝蛋白高(5 mg/kg)、中(3 mg/kg)、低(1 mg/kg)剂量组,尾静脉给药,1次/d,共8 d。停药次日取血后处死小鼠,计算抑瘤率,以酶联免疫法测定小鼠血清IL-4和IFN-y含量。结果:雷丸菌丝蛋白高、中、低剂量均能有效抑制肿瘤的生长(P<0.05)。高剂量抑制率达36.4%,但低于环磷酰胺43.2%的肿瘤抑制率。雷丸菌丝蛋白在抑制肿瘤生长的同时,能够增加小鼠血清IFN-γ含量,降低血清IL-4含量,并能提高脾脏指数。但对小鼠胸腺、肝脏、心脏、肺脏和肾脏指数无明显影响。结论:雷丸菌丝蛋白对H_(22)肝癌细胞所致实体瘤具有显著抑制作用,并能在一定程度上增强小鼠免疫功能。Objective：To explore the effect of proteins extraceed from mycelia of Omphalia lapidescens on inhibiting H22 liver cancer in vivo.Methods：50 hepatoma 22 tumor bearing mice models were divided into five groups randomly：control group（CG）,cyclophosphamide group,and 3 groups of incremental Hepatoma 22 dosages（5,3,1 mg/kg）.All groups were i.v.with drugs once a day.After 8 consecutive days,the concentrations of interferon-γ（IFN-γ） and interleukin-4（IL-4） in serum were detected by enzyme-linked immunoabsorbent assay（ELISA）.The weight changes of tumor,thymus,liver,heart,spleen,lung and kidney were observed.Results：It showed the tumors＇ weight were significant heavier in CG than in EGs.The tumor-inhibition rate（IR） was 36.4%in high dosage group,which was lower than 43.2%in cyclophosphamide group.The spleen mass of proteins groups increased significantly.The concentration of IFN-γin serum of proteins groups increased as CG,but IL-4 in inverse direction.The observations of thymus,liver,heart, lung and kidney in EGs were the same as CG.Conclusion：The proteins extracted from mycelium of Omphalia lapidescens can inhibit the growth of tumour and enhance the immune function of H22 tumor-bearing mice.

关 键 词：药用真菌 细胞因子 液体发酵 中药 

分 类 号：R285.5[医药卫生—中药学]