胆固醇氧化酶高产菌株的诱变选育及产酶条件优化  

Screening of high-yield strains of cholesterol oxidase by ultraviolet mutation and optimization of fermentation conditions

作　　者：黄寅[1,2] 葛飞[1] 陶玉贵[1] 汤斌[1] 李英[1] 

机构地区：[1]安徽工程科技学院生化工程系,安徽芜湖241000 [2]安徽工程科技学院微生物发酵安徽省工程技术研究中心,安徽芜湖241000

出　　处：《安徽工程科技学院学报（自然科学版）》2009年第4期8-11,共4页Journal of Anhui University of Technology and Science

基　　金：安徽高校自然科学基金资助项目(kj2009B071);安徽工程科技学院引进人才科研启动基金资助项目(2007yq005);芜湖市科技计划基金资助项目(2008505)

摘　　要：在对细脚拟青霉、玫烟色拟青霉、粉拟青霉、香菇、平菇、金针菇等胆固醇氧化酶产生菌初筛的基础上,选择以产酶较高的玫烟色拟青霉作为紫外诱变的出发菌株,筛选出一株相对高产突变菌株MFEC006,其酶活达到了0.548 3 U/mL,比出发菌株提高了154%,经8次传代培养,突变株性质稳定.对MFEC006菌株产酶条件进行优化,得出其最佳产酶条件为:pH为6.5,温度27℃,接种量10%,摇床转速180 r/min.On the basis of screening of Paecilomyces tenuipes, Paecilomyces fumosoroseus, Paecilomyces farinosus, Pleurotus ostreatus, Lentinus edodes and Flammulina velutipes, this paper chooses Paecilomyces fumosoroseus as original strain of UV treatment. The cholesterol oxidase producing mutant strain MFEC006 was obtained from Paecilomyces fumosoroseus, the enzyme activity reached 0. 548 3 U/mL, 154% higher than that of the starting strain. The properties of the mutant strain were stable after eight generations. The optimal fermentation conditions of strain MFEC006 were also studied. The results showed that the optimal culture conditions were as follows： pH 6.5, temperature 27 ℃, inoculum 10% and rotation rate 180 r/min.

关 键 词：胆固醇氧化酶 玫烟色拟青霉 紫外诱变 

分 类 号：Q936[生物学—微生物学]