地塞米松对过氧化氢体外氧化杀伤烟曲霉的影响  

The effects of dexamethasone on hydrogen peroxide oxidative killing of Aspergillus fumigatus in vitro

作　　者：许辉[1] 陈伟[1] 李丽娟[1] 万喆[1] 王晓红 李若瑜[1] 刘伟[1] 

机构地区：[1]北京大学第一医院皮肤性病科北京大学真菌和真菌病研究中心,北京100034

出　　处：《中国真菌学杂志》2009年第6期334-337,共4页Chinese Journal of Mycology

基　　金："十一五"科技部"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2008ZX10401);教育部科学技术研究重点项目(107002)

摘　　要：目的研究地塞米松对过氧化氢(H2O2)体外杀伤烟曲霉(Aspergillus fumigatus,A.fumigatus)的影响。方法用不同浓度的地塞米松(0 mg/mL,0.02 mg/mL,0.2 mg/mL)处理烟曲霉孢子,按照处理时间不同分为A(0.5 h),B(2 h),C(7 h),D(16 h)4组。用测定H2O2杀伤真菌的标准方法——斑点法分别测定各组烟曲霉孢子对H2O2的氧化杀伤敏感性。结果在H2O2浓度为1.5 mmol/L下,未用地塞米松处理的孢子(阴性对照)氧化杀伤敏感性为5×101(生长良好)。A(0.5 h)组:地塞米松0.02 mg/mL处理后孢子的氧化杀伤敏感性为5×103;地塞米松0.2 mg/mL处理后孢子的氧化杀伤敏感性为5×104。B(2 h)组:地塞米松0.02 mg/mL和0.2 mg/mL处理后孢子的氧化杀伤敏感性均为5×103。C(7 h)和D(16 h)组:不同浓度地塞米松处理后氧化杀伤敏感性与阴性对照没有差别(均为5×101)。结论地塞米松使H2O2在体外杀伤烟曲霉的能力增强,这种作用仅发生在地塞米松接触烟曲霉孢子的早期(<2 h)。Objective To study the effects of dexamethasone on hydrogen peroxide （H2O2 ） oxidative killing of Aspergillus fumigatus （A. fumigatus） in vitro. Methods A. fumigatus spores were administered with different concentrations （0 mg/mL,0.02 mg/mL,0.2 mg/mL） of dexamethasone and were divided into four groups （ A ：0.5 h, B ：2 h, C ：7 h, D ： 16 h） according to the treatment time. The H202 oxidative killing assay was performed, using spot test in the four groups. Results With 1.5 mmol/L of H2O2, the oxidative killing susceptibility of A. fumigatus without dexamethasone （ negative control） was 5 × 10^1 （ grow well）. Group A： the oxidative killing susceptibility of A.fumigatus treated with 0. 02 mg/mL of dexamethasone was 5 × 10^3 ; and that with 0.2 mg/mL of dexamethasone was 5 × 10^4. Group B ： the oxidative killing susceptibility of A.fumigatus treated with 0. 02 mg/mL and 0.2 mg/mL of dexamethasone was all 5 × 10^3. Group C and Group D： the oxidative killing susceptibility of A.fumigatus with different concentration of dexamethasone had no difference with that of negative control （5 × 10^1 ）. Conclusions Dexamethasone strenthens the ability of H2O2 to kill A. fumigatus in vitro , which happens only at the early stage （ 〈 2 h） of contacting with A. fumigatus spores.

关 键 词：烟曲霉 地塞米松 氧化杀伤 

分 类 号：R977.11[医药卫生—药品]