用精浆中性α-1,4-葡萄糖苷酶测定对无精子症病因的初步分析  

A preliminary analysis of the pathogeny of azoospermia by using determination of the activity of neutral alpha-1,4-glucosidase in seminal plasma

作　　者：罗比[1] 王萍[1] 陈复[1] 

机构地区：[1]四川省计划生育科学研究所生化室,成都610041

出　　处：《男科学报》1998年第3期185-188,共4页

摘　　要：目的：期望建立判断无精子症病因的诊断系统及诊断流程模型。方法：采用自制试剂盒对22例已知血清FSH水平的无精子症病人进行了糖浆中性α-1.4-葡萄糖苷酶活性测定。结果：与正常值（30.72±4.31U／Lx±2s）比较，无精子症病人的精浆酶活性显著减少（10.82±2.79U／L，x±2s），其中低于17U／L者有17例，占试验组总数的77％，揭示在无精子症中附睾功能损害或输精管道梗阻病变相当广泛、血清FSH测定表明.41％的病人可能有睾丸生精功能障碍、附睾特异性酶和激素测定结果的双向分类分析发现，以上指标均为正常或均为异常者各有2例和6例，分别占9％和27％.证实了无精子症病因的离散性、结论：根据初步的分析结果，提出了包括激素-酶-病理学检查手段在内的判断无精子症病因的诊断系统的构想。Objectives: In order to get better diagnosis of azoospermia, a preliminary thought of flow chart, a system comprising hormonal, enzymatic, and pathological examinatins, was proposed based on the results of this study. Methods: Neutral alpha-1, 4-glucosidase in seminal plasma from 22 cases of azoospermic patients,of which the level of serum FSH was known,had been determined by using our self--made kit. Results: The activity of this enzyme in seminal plasma from azoospermic patients was 10. 82±2.79U/L (x±2s ), being decreased significantly compared with the normal value (30. 72±4. 31U/L ). The case number of the patients whose enzyme level was less than 17U/Lwas seventeen and was 77 % of the total number of the experiment group,showing the fact that epididymal function defects and/or obstructive diseases in male reproductive tract are common in patients with azoospermia. The results of serum FSH showed that 41 % of the patients may be in trouble in their spermatogenesis. Conclusions: According to the results of a two--way clasification on the data of both epldidymal specific enzyme and hormone,the patients with all normal values and that with all abnormal ones was two and six (9% and 27 %), respectively,indicating the divergency of the pathogeny of azoospermia.

关 键 词：精浆 无精子症 FSH α-1 4-G 病因 

分 类 号：R698.202[医药卫生—泌尿科学]